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Nowadays cell-based assays have become an indispensible method in early stage drug discovery programs. Cell-based assays are widely used not only in drug discovery but also in toxicology, drug metabolism and pharmacokinetics (DMPK), release testing for therapeutic proteins etc.
Creative Biogene offers a growing number of ready-to-assay cells that are optimized and can be used for your drug discovery and development studies. Our ready-to-assay cells do not need culturing. It is a time/cost-effective option if you want to bypass cell culture or if you have a pilot scale experiment.
Features
The cells to be tested have a wide range of applications, mainly in the fields of biological and medical research. Here are some key uses:
Case Study 1
Centipede is a commonly used traditional Chinese medicine that has been shown to have anti-cancer effects. In this study, the inhibitory effect of centipede alcohol extract (AECS) was more prominent when treating cells that highly express epidermal growth factor receptor (EGFR) (A431 and HEK293/EGFR cells and HEK293 cells). The elution profile of AECS on a cell membrane chromatography (CMC) column indicates that AECS can bind to EGFR, and competition studies indicate that AECS and gefitinib may directly compete at a single common binding site on EGFR. In cell culture systems, AECS significantly induced apoptosis in A431 and HEK293-EGFR cells, which was associated with effects on the Bcl-2 family. Furthermore, AECS can alter EGFR kinase activity and reduce phosphorylation of EGFR and downstream signaling factors AKT and Erk1/2. The mechanism by which AECS inhibits proliferation of high EGFR-expressing cells is due to its ability to induce apoptosis and modulate the EGFR pathway. This research may provide a new treatment for cancers with high EGFR expression.
Figure 1. Effect of AECS on the growth of cells with high EGFR expression. Cells were treated with AECS at indicated concentration for 24, 48 and 72 h. Cell growth was measured by MTT. (A) HEK293 cells. (B) HEK293-EGFR cells. (C) A431 cells. (D) The expression levels of EGFR in HEK293, HEK293-EGFR, and A431 cells without any treatment were examined by Western blotting assay, and the results were quantified by densitometric analysis of the bands and GAPDH normalization. Cells were treated with different concentrations of AECS. Cell growth was measured after 48 hours by MTT (E) and trypan blue staining assay (F). (Ma W, et al., 2015)
Case Study 2
Exosomes are extracellular vesicles that mediate intercellular communication by transferring biological materials such as DNA, RNA, and proteins. In this study, researchers explored the potential of single-chain variable fragments (scFv) as targeting domains to enable exosome delivery to cells expressing cognate antigens. The researchers generated exosomes that targeted the Her2 receptor by altering the affinity of the scFv and the level of Her2 expression on the receptor cells. They determined that high-affinity anti-Her2-scFv and cells expressing high levels of Her2 were optimal conditions to achieve selective uptake. Furthermore, targeting exosomes to cells via specific cell surface receptors can alter their intracellular trafficking pathways, providing the opportunity to influence the delivery efficiency and fate of intracellular cargo. These experiments provide robust data supporting the broader application of exosomes displaying antibody fragments as vehicles for targeted delivery of therapeutic molecules.
Figure 2. High expression of Her2 is required for efficient targeting of exosome uptake. (A) Quantification of absolute Her2 receptor numbers on three clonal HEK293 cell lines HEK-Her2-L, HEK-Her2-M, and HEK-Her2-H and parental HEK cells by flow cytometric analysis using standardized beads. Receptor numbers were calculated by extrapolation to a standard curve. (B) and (C) Parental HEK293 and HEK-Her2 overexpression cell lines were incubated in duplicate with wild-type, non-targeted, and targeted CFSE exosomes for 4 h, and cells were analyzed by flow cytometry. (D) Parental HEK293 and HEK-Her2 overexpression cell lines were incubated with exosomes and then stained with WGA-Alexa-flor555 (red) and Hoechst (blue) after fixation. (Longatti A, et al., 2018)
Q: What are Ready-To-Assay Cells?
A: Ready-to-assay cells are cells that have been genetically modified to express various targets and can be tested immediately upon receipt. These cells are particularly suitable for high-throughput screening and functional assays to facilitate drug discovery and biological research.
Q: What is the purpose of using Ready-To-Assay Cells?
A: Ready-to-use cells are used to accelerate research in areas such as drug discovery, toxicology research, and cell biology. Because the cells are ready for use upon arrival, cell culture is not required, saving valuable time and resources.
Q: How are Ready-To-Assay Cells produced?
A: Ready-to-assay cells are produced by transfecting cells with a specific gene encoding the desired protein target. The cells are then grown in culture until the desired density is reached. They are then harvested, counted and stored frozen until needed.
Q: What are the benefits of using Ready-To-Assay Cells over traditional cell cultures?
A: The main advantage of using ready-to-assay cells is significant time and labor savings. Traditional cell culture requires a significant investment of resources and time, and a certain level of expertise is required to maintain the cells. In contrast, ready-to-assay cells can be handled even by non-experts and can be used immediately upon receipt, streamlining workflow.
Q: Can Ready-To-Assay Cells be used for all types of assays?
A: Not all assays are available with Ready-To-Assay Cells. However, these cells are extremely useful for a wide range of functional analyses, including those for drug discovery and biological research. Their suitability for a specific assay depends largely on the nature of the specific target protein being studied.
| Cat.No. | Product Name | Price |
|---|---|---|
| RTA-0001 | Jurkat-CD16/Dual-Luc Effector Assay Cells | Inquiry |
| RTA-0002 | HEK293-HER2 (+) Assay Cells | Inquiry |
| RTA-0003 | HEK293-HER2 (-) Assay Cells | Inquiry |
| RTA-0004 | HEK293-mTNF-alpha (+) Assay Cells | Inquiry |
| RTA-0005 | HEK293-mTNF-alpha (-) Assay Cells | Inquiry |
| RTA-0006 | HEK293-EGFR (+) Assay Cells | Inquiry |
| RTA-0007 | HEK293-EGFR (-) Assay Cells | Inquiry |
| RTA-0008 | K562-TNFalpha Assay Cells | Inquiry |
| RTA-0009 | U937-Type I IFN Assay Cells | Inquiry |
| RTA-0010 | DT40-Insulin Assay Cells | Inquiry |
| RTA-0011 | HEK293-FGF21 Assay Cells | Inquiry |
| RTA-0012 | DT40-IL2 Assay Cells | Inquiry |
| RTA-0013 | HEK293-IL6 Assay Cells | Inquiry |
| RTA-0014 | DT40-IL23 Assay Cells | Inquiry |
| RTA-0015 | DT40-IL12 Assay Cells | Inquiry |
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