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miRNAs (microRNAs) are essential regulators of target mRNA expression, playing pivotal roles in numerous developmental and physiological processes. These molecules undergo processing from primary transcripts (pri-miRNA) to pre-miRNA, ultimately maturing into functional miRNA forms. Creative Biogene offers an extensive array of miRNA expression plasmids encompassing all miRNAs listed in the miRbase database. Our plasmids ensure robust miRNA expression and enable users to monitor expression using a reporter gene. Explore our high-quality miRNA plasmids to discover the perfect match for your research needs. Our vector-based miRNA expression plasmids simplify experimental complexities, facilitating down-regulation of gene expression. If your gene of interest is not available in our collection, we offer customized solutions at competitive prices.
Key Features of Our miRNA Expression Plasmids
Creative Biogene offers a wide range of miRNA expression plasmids designed to facilitate research in miRNA biology and gene regulation. These plasmids are valuable tools for studying the functions of specific miRNAs, investigating their roles in cellular processes, and exploring their potential as therapeutic targets. Researchers can utilize Creative Biogene's miRNA expression plasmids to:
Case Study 1
Drug addiction's persistent and experience-dependent nature may involve epigenetic changes, including non-coding miRNAs, crucial for neuronal function and modulated by cocaine in the striatum. Researchers aimed to investigate the role of specific miRNAs, modulated by cocaine, in striatal cell populations and their contribution to drug addiction. By evaluating the expression of selected miRNAs in the nucleus accumbens (NAc) and dorsal striatum (DS) upon sustained cocaine exposure in mice, they found preferential upregulation in the NAc. Focusing on miR-1 and its predicted mRNA targets, Fosb and Npas4, they validated these targets both in vitro and in vivo. Utilizing RNA cloning techniques, they explored the potential of miR-1 to regulate cocaine-induced behavior by overexpressing it in specific striatal cell populations. In DS D1-SPNs, miR-1 overexpression downregulated Fosb and Npas4, reducing cocaine-induced CPP reinstatement but increasing cue-induced cocaine seeking. In DS D2-SPNs, miR-1 overexpression reduced the motivation to self-administer cocaine.
Figure 1. Researchers utilized RNA clones to experimentally validate the direct targeting of FosB and Npas4 mRNA 3'UTRs by miR-1. The pmirGLO-3′UTR_Bdnf, pmirGLO-3′UTR_FosB, and pmirGLO-3′UTR_Npas4 plasmids were commercially obtained from Creative Biogene in this study. (Forget B, et al., 2022)
Case Study 2
MiRNAs inhibit protein expression by binding to target mRNAs. Researchers observed a coordinated biogenesis of miRNAs in activated macrophages, where miR-146a-5p serves as a "primary" miRNA inducing the expression of "secondary" miRNAs such as miR-125b, miR-21, or miR-142-3p. This coordinated biogenesis balances immune responses by targeting new sets of mRNAs. The use of miRNA Expression Plasmids allows researchers to explore the regulatory roles of miRNAs and manipulate their expression to study their impact on cellular processes such as immune response modulation. Inhibition of the "primary" miR-146a-5p-specific inhibitor disrupts the coordinated biogenesis of secondary miRNAs, providing insights into potential therapeutic strategies for controlling cytokine expression and inflammatory responses in activated macrophages.
Figure 2. The regulatory role of miR-146a-5p in the biogenesis of miRNAs in LPS-induced murine macrophages was investigated. Anti-miR-146a oligonucleotides were utilized to inhibit miR-146a-5p activity, and the expression levels of miRNAs, including miR-125b, miR-142-3p, and miR-21, along with their precursors and associated proteins, were examined. (Chatterjee S, et al., 2022)
Q: What is the range of miRNAs covered in your miRNA Expression Plasmids collection?
A: Our miRNA Expression Plasmids cover all miRNAs listed in the miRbase database, providing researchers with a comprehensive selection to suit various experimental needs. This extensive range ensures that customers can find the specific miRNA they require for their studies, enhancing the versatility of our products.
Q: How do you ensure strong miRNA expression in your plasmids?
A: Creative Biogene leverages years of experience and expertise to guarantee robust miRNA expression in our plasmids. We utilize advanced vector-based technologies and rigorous quality control measures to optimize miRNA expression levels, ensuring reliable and reproducible results for our customers' experiments.
Q: Can users track miRNA expression in their experiments?
A: Yes, our miRNA Expression Plasmids are designed to enable users to track miRNA expression using a reporter gene. This feature allows researchers to monitor miRNA expression levels and assess the efficacy of their experiments accurately. By providing this capability, we empower customers to optimize their experimental workflows and obtain meaningful data with ease.
Q: Are your miRNA Expression Plasmids ready to use upon purchase?
A: Absolutely, our miRNA Expression Plasmids are purified and ready for transfection upon purchase. This convenience saves researchers valuable time and effort in preparing their experimental materials, allowing them to focus more on their research objectives. Additionally, our ready-to-use plasmids undergo stringent quality control processes to ensure optimal performance, enhancing the reliability of experimental outcomes.
| Cat.No. | Product Name | Price |
|---|---|---|
| MIPH2001 | MIRLET7A1-pCMV-MIR | Inquiry |
| MIPH2002 | MIRLET7A2-pCMV-MIR | Inquiry |
| MIPH2003 | MIRLET7A3-pCMV-MIR | Inquiry |
| MIPH2004 | MIRLET7B-pCMV-MIR | Inquiry |
| MIPH2005 | MIRLET7c-pCMV-MIR | Inquiry |
| MIPH2006 | MIRLET7d-pCMV-MIR | Inquiry |
| MIPH2007 | MIRLET7e-pCMV-MIR | Inquiry |
| MIPH2008 | MIRLET7f-1-pCMV-MIR | Inquiry |
| MIPH2009 | MIRLET7f-2-pCMV-MIR | Inquiry |
| MIPH2010 | MIR15a-pCMV-MIR | Inquiry |
| MIPH2011 | MIR16-1-pCMV-MIR | Inquiry |
| MIPH2012 | MIR17-pCMV-MIR | Inquiry |
| MIPH2013 | MIR18a-pCMV-MIR | Inquiry |
| MIPH2014 | MIR19a-pCMV-MIR | Inquiry |
| MIPH2015 | MIR19B-1-pCMV-MIR | Inquiry |
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