Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | T24 is an important human epithelial cell line derived from highly malignant and aggressive bladder transitional cell carcinoma. Initially established in an 81-year-old female patient, it is one of the earliest and most extensively studied human cancer cell lines. A notable biological characteristic of the T24 cell line is the presence of a mutated and activated HRAS (Ha-ras) oncogene, specifically a point mutation at amino acid position 12. These cells have a generation time of approximately 19 hours and are known for their high motility. While the original T24 cell line has shown limited tumorigenicity in nude mice, its derived sublines, such as T24T, have been developed to mimic advanced metastatic progression and higher malignancy. |
| Tissue | Bladder |
| Disease | Bladder Carcinoma; Transitional Cell Carcinoma (TCC) |
| Morphology | Epithelial-like |
| Gender | Female |
| Age | 81 years |
| Ethnicity | Caucasian |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 |
| Applications | 1. Bladder Cancer Biology: 2. Oncogene and Signaling Research 3. Drug Screening and Chemosensitivity 4. Apoptosis and Cell Cycle Studies 5. Cytokine and Autocrine Signaling |
| Shipped In | Dry ice |
| Storage Temperature | Below −140°C or in liquid nitrogen vapor phase |
| Characteristics | |
| Karyotype | Hypertriploid to hypopentaploidy; modal chromosome number is 82 (range 82-132). Features distinctive marker chromosomes, including metacentrics and complex interchromosomal exchanges. |
| Tumorigenic | Yes (in hamster cheek pouch); No (original T24 in nude mice). Malignant transformation can be induced chemically. |
| Mutations | Homozygous HRAS mutation; Homozygous TP53 mutation; TERT promoter mutation. |
| Antigen Expression | HLA A1, A3, B18, Bw35, Cw4, DRw2, Dw4. |
| Isoenzymes | G6PD type B; AK-1, 1; ES-D, 1; GLO-I, 1; Me-2, 1-2; PGM1, 1; PGM3, 1. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Gently rinse the cell layer with calcium- and magnesium-free PBS buffer or 0.25% trypsin-EDTA solution to remove residual serum. 3. Add 1.0 to 3.0 mL of 0.25% (w/v) trypsin-0.53 mM EDTA solution (for T75 culture flasks). 4. Observe under an inverted microscope until the cell layer disperses (usually 2 to 10 minutes). For cells that are difficult to separate, incubate at 37°C to promote detachment. 5. Add an equal volume of complete culture medium to neutralize the trypsin. 6. Gently pipette the cells and transfer them to centrifuge tubes. 7. Centrifuge at 125 x g for 5-7 minutes. 8. Resuspend the cell pellet in fresh complete culture medium and aliquot into new culture containers. |
| Medium Renewal | 2 to 3 times per week. |
| Subcultivation Ratio | A split ratio of 1:3 to 1:8 is recommended. |
| Culture Medium | McCoy's 5a Medium Modified + 10% Fetal Bovine Serum (FBS). Alternatively, DMEM/F12 (1:1) + 5-10% FBS. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO. |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO0182 | Cas9 Stable Cell Line - T24 | Inquiry |
| CSC-RR00717 | Luciferase Reporter Cell Line - T24 | Inquiry |
| CSC-RR00874 | GFP/Luc Reporter Cell Line - T24 | Inquiry |
| CSC-RR01044 | mScarlet/LUC Reporter Cell Line - T24 | Inquiry |
| CSC-RR01111 | AP1-Luc/EF1α-mCherry Reporter Cell Line - T24 | Inquiry |
| CSC-RR01112 | ARE-Luc/EF1α-mCherry Reporter Cell Line - T24 | Inquiry |
| CSC-RR01113 | ISRE-Luc/EF1α-mCherry Reporter Cell Line - T24 | Inquiry |
| CSC-RR01114 | Myc-Luc/EF1α-mCherry Reporter Cell Line - T24 | Inquiry |
| CSC-RR01115 | NFκB-Luc/EF1α-mCherry Reporter Cell Line - T24 | Inquiry |
| CSC-RR01116 | p53-Luc/EF1α-mCherry Reporter Cell Line - T24 | Inquiry |
| CSC-RR01117 | STAT3-Luc/EF1α-mCherry Reporter Cell Line - T24 | Inquiry |
| CSC-RR01118 | IL6pro-Luc/EF1α-mCherry Reporter Cell Line - T24 | Inquiry |
| CSC-RR01119 | TNFαPro-Luc/EF1α-mCherry Reporter Cell Line - T24 | Inquiry |
Copyright © Creative Biogene. All rights reserved.