MKN45 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	MKN45 cells are a human gastric cancer cell line that was established from biopsy specimens from patients with gastric adenocarcinoma. These cells are widely used as an in vitro model of gastric cancer to study the mechanisms underlying tumor development and to test the efficacy of potential therapeutic agents. MKN45 cells exhibit many characteristics of gastric cancer cells, including the ability to form colonies in vitro and to form tumors when injected into immunocompromised mice. MKN-45 cells are epithelial in nature and grow as adherent cells in culture. They express various biomarkers associated with gastric cancer, such as carcinoembryonic antigen (CEA) and E-cadherin, making them useful for diagnostic and therapeutic studies. In addition, MKN-45 cells are often used to evaluate chemotherapeutic agents and targeted therapies because they respond to treatment and are able to mimic the clinical behavior of human gastric tumors.
Tissue	Stomach
Disease	Gastric adenocarcinoma
Morphology	Epithelial-like
Gender	Female
Age	62 years
Product Format	Frozen
Growth Mode	Adherent/suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer research 2. Drug testing and development 3. Molecular pathway studies 4. Gene expression analysis 5. Genetic studies 6. Tumor microenvironment studies 7. In vivo models
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	MKN-45 cells have been used to study the role of various signaling pathways in gastric cancer, including the Wnt/β-catenin, PI3K/Akt, and MAPK/ERK pathways. These pathways are known to be dysregulated in a variety of cancers, including gastric cancer, and targeting them with therapeutic agents has shown promise in preclinical studies. MKN-45 cells have also been used to test the efficacy of various chemotherapeutic agents, including cisplatin, 5-fluorouracil, and docetaxel.
Characteristics
Karyotype	Human hypertriploid karyotype with 8% polyploidy
Tumorigenic	Yes, in nude mice.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove old medium from adhered cells and wash with PBS without calcium and magnesium. For T25 flasks, use 3-5 ml PBS and for T75 flasks, use 5-10 ml. 2. Then completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow the cells to incubate for 10 minutes at room temperature. 4. After incubation, combine the suspended and adherent cells and centrifuge. 5. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension to a new flask containing fresh medium.
Subcultivation Ratio	The ratio of 1:3 to 1:5 is recommended.
Culture Medium	80% RPMI 1640 + 20% h.i. FBS
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%; Temperature: 37°C
Cryopreservation	Frozen with 70% medium, 20% FBS, 10% DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

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* For research use only. Not intended for any clinical use.

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