Plant RNA Fluorescent In Situ Hybridization (FISH) Service

RNA Fluorescent In Situ Hybridization (FISH) is a widely-applicable histology technique that utilizes a nucleic-acid based probe to localize to RNA sequences of interest, and allows for visualization of RNA expression in cells or tissues. This technique can provide valuable special and temporal information regarding gene expression in situ, such as whether expression at the mRNA level is limited to a specific anatomic structure or cell type. And with RNA FISH assays you can also visualize small RNA (siRNA or ASOs), microRNAs and lncRNAs. Creative Biogene offers Custom Plant RNA FISH Services.

Figure 1. A schematic representation of Z probe-based RNAScope assay for lncRNA analysis. (Tripathi MK, et al., 2018)

In the plant systems, the FISH assays have been employed in multiple crop species, including wheat, rye, cucumber and melon. With solid biochemical and cytological backgrounds, and years of experience in plant cell line/tissue sample generation, scientists and experts here at Creative Biogene provide high quality service that covers every step of the RNA FISH assay in the plant system.

Figure 2. Arabidopsis thaliana leaf tissue. Probe 1 of interest (Red). Probe 2 of interest (Green). DAPI (Blue)

Figure 3. Single-plex detection in RNAscope V2 assay for HvGAPDH mRNA on barley line Q21861 leaf sections during assay optimization.

Advantages of Plant RNA Fluorescent In Situ Hybridization (FISH) Service

Specificity- RNA FISH enables detecting specific RNA molecules at subcellular resolution, which allows accurate and specific localization of target RNAs in their cellular context.
Single-Cell Resolution- This technique offers single-cell resolution and can be used to analyze the spatial organization of RNA within tissues and cells.
Direct Visualization- RNA FISH provides direct visualization of RNA molecules, giving a more precise insight into cellular processes.
Quantification- This technique allows quantification of individual RNA molecules in cells and tissues, enabling detailed studies of gene expression at the single-cell level.
High Sensitivity- RNA FISH is a highly sensitive method, allowing detection of low-abundance transcripts.
Value- We focus on the quality of our service and all supported by competitive pricing
Efficiency- We are able to provide the fastest turnaround time of any supplier in the industry

Creative Biogene offers Plant RNA Fluorescent In Situ Hybridization (FISH) Service for your scientific research as follows:

Probe design (Or use ACD RNAscope® Probe)
Probe synthesis (Or use ACD RNAscope® Probe)
Sample (Cells/FFPE/Others) preparation
RNA Fluorescent In Situ Hybridization
Imaging
Data analysis

Quotation and ordering

Our customer service representatives are available 24hr a day! We thank you for choosing Creative Biogene at your preferred Plant RNA Fluorescent In Situ Hybridization (FISH) Service.

* For research use only. Not intended for any clinical use.

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