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Creative Biogene offers a comprehensive selection of full-length ORF clones, omitting 5' and 3' UTRs for expedited protein expression. These ORFs facilitate various downstream applications like protein detection and purification, aiding researchers in unraveling intricate biological processes. Leveraging advanced cloning technologies, our extensive ORF clone library covers diverse species, enabling researchers to locate genes of interest efficiently. With our sequence-verified ORFs, Creative Biogene accelerates research endeavors, empowering scientists to delve deeper into their investigations. Explore our ORF collection to discover the ideal clone for your research needs.
Advantages of Our ORF Clones:
Creative Biogene can accelerate your research by providing sequence-verified human ORFs. Please browse our human ORF clones collection to find your desired one.
Creative Biogene offers the largest collection of ORF clones, with genome-wide coverage for mice.
Creative Biogene offers the largest collection of ORF clones, with genome-wide coverage for rats.
Creative Biogene, as one of the leading biotechnology companies, is able to offer a comprehensive selection of Cynomolgus ORF clones.
Creative Biogene can accelerate your research by providing sequence-verified canine ORFs. Please browse our canine ORF clones collection to find your desired one.
Combining unique and versatile cloning technologies, Creative Biogene has created a large number of high-quality rabbit ORF clones where you can find your gene of interest.
Creative Biogene has created a large number of high-quality Chicken ORF clones where you can find your gene of interest.
Creative Biogene has created a large number of high-quality zebrafish ORF clones where you can find your gene of interest. Please browse our collection of zebrafish cDNA clones to find the one you want.
Combining unique and versatile cloning technologies, Creative Biogene has created a large number of high-quality ferret ORF clones.
Combining unique and versatile cloning technologies, Creative Biogene has created a large number of high-quality SARS-CoV-1 ORF clones. Premade ORF clones eliminate the initial cloning and validation procedures for gene discovery studies.
Creative Biogene, as one of the leading biotechnology companies, is able to offer a comprehensive selection of SARS-CoV-2 ORF clones to assist the researchers to accelerate your pursuit to better understand SARS-CoV-2 and to ultimately conquer it.
Creative Biogene, as one of the leading biotechnology companies, is able to offer a comprehensive selection of Zika Virus ORF clones to assist the researchers to accelerate your pursuit to better understand Zika and to ultimately conquer it.
Creative Biogene, one of the leading biotechnology companies, offers a comprehensive selection of MERS-CoV ORF clones.
HIV ORF clones can help create a detailed map of viral gene function and how they interact with host cell machinery.
Combining unique and versatile cloning technologies, Creative Biogene has created a large number of high-quality HCV ORF clones.
Combining unique and versatile cloning technologies, Creative Biogene has created a large number of high-quality HeV ORF clones.
Combining unique and versatile cloning technologies, Creative Biogene has created a large number of high-quality NiV open reading frame (ORF) clones.
Creative Biogene has created a large number of high-quality SFTSV ORF clones. Premade ORF clones eliminate the initial cloning and validation procedures for gene discovery studies.
Creative Biogene can accelerate your research by providing sequence-verified P. falciparum ORFs. Please browse our P. falciparum ORF clones collection to find your desired one.
Creative Biogene has created a large number of high-quality DENV ORF clones. Premade ORF clones eliminate the initial cloning and validation procedures for gene discovery studies.
Creative Biogene has created a large number of high-quality WNV ORF clones. Premade ORF clones eliminate the initial cloning and validation procedures for gene discovery studies.
cDNA and ORF clones offer a rapid and readily accessible approach for acquiring gene content suitable for various in vitro and cell culture-based overexpression assays. The coding sequence is inserted into an expression vector governed by a potent promoter, and subsequently introduced into target cells through plasmid transfection or lentiviral transduction methodologies. ORF clones are tailored for protein-centric research, whereas cDNA clones offer a broad range of applications, including gene expression analysis and functional genomics research. Our ORF clones facilitate the following research endeavors:
Case Study 1
The development of the eye lens relies on the ongoing process of transforming lens epithelial cells into lens fiber cells. Researchers aimed to investigate the role of hypoxia and HIF1a in the degradation of organelles during lens fiber cell formation. They utilized ex vivo cultured embryonic chick lenses to monitor the degradation of mitochondria, Golgi apparatus, and endoplasmic reticulum under low (1% O2) and high (21% O2) oxygen conditions. Additionally, they examined the requirement for HIF1a activation using specific activators and inhibitors, as well as the transcriptional regulation of BNIP3L, known to be involved in organelle elimination. By expressing an oxygen-insensitive mutant form of HIF1a and BNIP3L, they demonstrated the essential role of hypoxia and HIF1a in organelle degradation and the attainment of mature lens structure. These findings shed light on the mechanisms underlying lens fiber cell differentiation and may have implications for understanding and treating ocular disorders.
Figure 1. Researchers analyzed protein extracts from fiber cell fractions to assess organelle content using specific markers via western blot analysis. Researchers obtained the Gallus gallus CMV-driven BNIP3L ORF and the Gallus gallus CMV-driven HIF1a P402A/P565A ORF from Creative Biogene. (Brennan L, et al., 2020)
Case Study 2
PDIs play crucial roles in cellular processes such as differentiation, function, and survival. Yet, comprehensively identifying and profiling sequence-specific PDIs in complex genomes remains challenging. Researchers employed ORF Clone to systematically explore the human protein-DNA interactome, aiming to identify and characterize sequence-specific protein-DNA interactions crucial for transcriptional regulation and cellular functions. Through a combined approach of bioinformatics and protein microarray, they unveiled 17,718 PDIs involving 460 DNA motifs and 4,191 human proteins, including both known and novel PDIs for transcription factors (TFs). Surprisingly, they discovered over three hundred proteins, previously not recognized as TFs, displaying sequence-specific PDIs, underscoring the diverse roles of proteins like RNA binding proteins, mitochondrial proteins, and protein kinases in transcriptional regulation.
Figure 2. Human ORFs were transferred from selected entry clones to a yeast expression vector (pEGH-A) using the recombinant cloning system. The resulting plasmids, producing GST-His6 fusion proteins, were confirmed by restriction endonuclease digestion and then transformed into yeast for protein purification. (Hu S, et al., 2009)
Q: What quality control measures are implemented for ORF clones?
A: Creative Biogene ensures the highest quality standards for ORF clones through rigorous sequence verification and validation procedures. Each clone undergoes comprehensive testing to verify its accuracy and functionality, ensuring reliable results in downstream applications.
Q: Are there options for customizing ORF clones to suit specific research needs?
A: Yes, Creative Biogene offers customizable options for ORF clones, allowing researchers to tailor the clones to their specific requirements. This includes selecting vector systems, adding tags, or incorporating regulatory elements as needed, providing flexibility and versatility for various experimental designs.
Q: How compatible are Creative Biogene's ORF clones with different expression systems?
A: Our ORF clones are designed to be compatible with a wide range of expression systems, including popular mammalian, bacterial, and yeast expression platforms. This versatility allows researchers to choose the most suitable system for their specific experimental needs, ensuring optimal expression and functionality of the cloned genes.
Q: Can Creative Biogene provide technical support and assistance for using ORF clones in experiments?
A: Yes, Creative Biogene offers comprehensive technical support and assistance to customers using ORF clones in their experiments. Our team of experts is available to guide experimental design, clone selection, troubleshooting, and data interpretation, ensuring successful outcomes for researchers utilizing our products.
| Cat.No. | Product Name | Price |
|---|---|---|
| CDCB156026 | Rat EGFL8 ORF clone (NM_001165880.1) | Inquiry |
| CDCB156028 | Rat CNBP ORF clone (XM_006236854.1) | Inquiry |
| CDCB156030 | Rat LDHB ORF clone (BC059149.1) | Inquiry |
| CDCB156034 | Rat MANBAL ORF clone (NM_001173380.1) | Inquiry |
| CDCB156036 | Rat SERPINB5 ORF clone (NM_057108.2) | Inquiry |
| CDCB156062 | Rat AKT2 ORF clone (DQ198085.1) | Inquiry |
| CDCB156065 | Rat BHMT ORF clone (BC078811.1) | Inquiry |
| CDCB156072 | Rat PLIN3 ORF clone (XM_001061015.1) | Inquiry |
| CDCB156078 | Cynomolgus RDH12 ORF clone (NM_001194401.1) | Inquiry |
| CDCB156082 | Cynomolgus TUBA1B ORF clone (NM_001285253.1) | Inquiry |
| CDCB156095 | Cynomolgus SUMO2 ORF clone | Inquiry |
| CDCB156109 | Cynomolgus ARF1 ORF clone | Inquiry |
| CDCB156129 | Cynomolgus HM13 ORF clone | Inquiry |
| CDCB156135 | Cynomolgus UBXN4 ORF clone (XM_005573040.1) | Inquiry |
| CDCB156156 | Cynomolgus DGCR6 ORF clone | Inquiry |
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