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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | The Neuro-2a cell line is derived from mice and has neuronal and amoeboid stem cell morphology, allowing it to differentiate in response to environmental factors. The differentiated cells possess many properties of neurons, including neurofilaments. Differentiation of Neuro-2a cells is caused by activation of the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) and phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) signaling pathways. Due to passage since initial collection, these cells can exhibit different responses to toxins than neuronal cells in living organisms. The Neuro-2a cell line also provides insights into the role of various genes and proteins in neuronal function and development. For example, the DNMT3A gene, known for its involvement in the DNA methylation process, has been studied in Neuro-2a cells to understand its effects on neuronal cells and neurodevelopmental processes. |
| Tissue | Brain |
| Disease | Neuroblastoma |
| Morphology | Neuronal and amoeboid stem cells |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Neural differentiation and function research 2. Drug discovery and toxicity testing 3. Study of neurological diseases 4. Genetic and molecular studies 5. Study of neuronal development 6. 3D cell culture 7. High-throughput screening |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Neuro-2a cells differentiate rapidly, reliably and easily, making them useful for research applications related to neurons and neuronal diseases. Serum withdrawal is a common method for inducing Neuro-2a cell differentiation and involves removing the serum on which the cells are growing to activate signaling pathways that control differentiation. Neuro-2a cells have been used to study neurite outgrowth, neurotoxicity, Alzheimer's disease, asymmetric division of mammalian cell lines, adenoviral transduction, and diagnosis of rabies. One specific research application is the differentiation of Neuro-2a cells into dopamine neurons for the treatment of Parkinson's disease. |
| Characteristics | |
| Karyotype | Mode = 95; Range = 59 to 193. The karyotype is unstable in the stem range of 94 to 98 chromosomes. All cells contain 6 to 10 large chromosomes with a median or submedian centromere and 2 to 4 microchromosomes. |
| Antigen Expression | H-2a |
| Virus Susceptibility | Herpes simplex virus Vesicular stomatitis virus Human poliovirus 1 |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old culture medium from adherent cells and wash with calcium- and magnesium-free PBS. For T25 flasks, use 3-5 ml of PBS and for T75 flasks, use 5-10 ml. 2. Then, completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow cells to detach by incubating at room temperature for 8-10 minutes. 4. After incubation, resuspend cells by gently mixing with 10 ml of medium and centrifuge at 300xg for 3 minutes. 5. Discard the supernatant, resuspend the cells in fresh medium, and transfer them to a new flask that already contains fresh medium. |
| Medium Renewal | 1 to 2 times per week |
| Subcultivation Ratio | The ratio of 1:4 is recommended. |
| Culture Medium | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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