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Recombinant adeno-associated viruses (AAVs) are crucial for noninvasive gene delivery, addressing the limitations of current methods. Creative Biogene, a leader in AAV technology, offers PHP.B, PHP.eB, and PHP.S capsids with superior tropism in the nervous systems. Overcoming AAV9 inefficiency, these capsids allow precise control of gene expression in challenging cell populations. Our quality-controlled AAV-PHP particles ensure efficient and advanced gene transfer, providing researchers with reliable tools for diverse applications. Explore our ready-to-use viral preparations to elevate your gene therapy and research endeavors.
The AAV-PHP capsids, developed through evolutionary selection, exhibit enhanced affinity for neuronal and glial cell receptors. Achieving exceptional neuronal tropism via non-invasive intravenous injection, these capsids overcome the limitations of natural ones, avoiding CNS damage. Engineered for safe and non-invasive gene therapies, these capsids offer innovative tools for neuroscience research. We currently offer transparent series products:
Case Study 1
AAV-PHP (Adeno-Associated Virus-PHP) capsid engineering marks a pivotal advance in central nervous system (CNS) research, enabling efficient gene expression modulation in the brain. These capsids, capable of crossing the blood-brain barrier, selectively transduce brain cells after systemic intravenous delivery, dependent on the Ly6a genotype. To assess efficiency, researchers utilized the Opera Phenix system with Harmony software, automating data segmentation into brain regions. Employing the AAV-PHP.B virus, the study rapidly and comparably quantified transduction efficiencies across the entire brain, cortex, striatum, and hippocampus. The results highlight consistent neuronal percentages and transduction rates, emphasizing the versatility of AAV in CNS research. This methodology, characterized by speed and reliability, provides a streamlined approach for assessing various parameters of interest through fluorescent signals.
Figure. 1 Mice brains immunostained after 3 weeks revealed even GFP distribution in C57BL/6J, confirming AAV-PHP.B-CAG-NLS-GFP crossed the BBB. In CBA/Ca mice, the GFP signal was absent, indicating distinct transduction responses. AAV proves crucial for brain gene expression insights. (Smith E J, et al., 2021).
Case Study 2
Adeno-associated virus (AAV) vectors have become a widely adopted, safe, and efficient means of delivering genetic material to the central nervous system (CNS). AAV-PHP.B and AAV-PHP.eB, variants derived from AAV9, exhibit broader cell transduction throughout the CNS compared to the original serotype, AAV9. In an evaluation involving rhesus macaque monkeys, lumbar intrathecal delivery of AAV-PHP.eB:CAG-EGFP demonstrated superior and widespread CNS transduction in comparison to AAV-PHP.B. Both variants showed robust tropism for neurons and oligodendroglial cells in the putamen and hippocampus. This proof-of-concept experiment underscores the potential of intrathecal AAV-PHP.eB infusions for precise and cell-type-specific distribution of genetic material within the CNS. This approach introduces a promising avenue for modeling brain diseases in rhesus macaques and advancing gene therapies targeting the human CNS, with AAV playing a pivotal role in these advancements.
Figure 2. AAV-PHP.eB: CAG-EGFP demonstrated superior GFP transduction efficiency in a pilot study with NHPs, confirming the potential of AAV9-derived variants for neural cell transduction after intrathecal delivery. Both monkeys tolerated the vectors well, and qualitative and quantitative assessments revealed extensive and intense EGFP expression in AAV-PHP.eB-injected monkey's CNS compared to AAV-PHP.B. (Arotcarena M L, et al., 2021).
Q: What is the significance of AAV PHP in gene delivery?
A: AAV PHP, or Engineered Adeno-Associated Viruses, is a specialized series of viral vectors designed for efficient and non-invasive gene delivery. These vectors are engineered to target specific tissues, with a primary focus on the central nervous system (CNS) and peripheral nervous system (PNS).
Q: How do I differentiate between various AAV PHP variants?
A: The choice of AAV PHP variants depends on the specific tissue or cell population you aim to target. Each variant, such as AAV PHP.B, AAV PHP.eB, and AAV PHP.S, possesses distinctive properties, tropism, and transduction efficiencies. Select the variant based on your experiment's requirements for optimal outcomes.
Q: In what scientific research areas can AAV PHP products be applied?
A: AAV PHP products find extensive applications in diverse scientific research realms. They are particularly valuable in neuroscience studies, gene therapy research, and investigations involving specific tissues like the CNS and PNS. These products serve as versatile tools for achieving precise gene expression and manipulation, contributing to a broad spectrum of research endeavors.
Q: How does each AAV PHP variant differ in terms of transduction efficiency and tropism?
A: Each AAV PHP variant, including AAV PHP.B, AAV PHP.eB, and AAV PHP.S, exhibits unique characteristics regarding transduction efficiency and tropism. Understanding these differences is crucial for selecting the most suitable variant based on the target tissue or cell population in your study.
| Cat.No. | Product Name | Price |
|---|---|---|
| AAV00116Z | AAV PHP.B-Null | Inquiry |
| AAV00117Z | AAV PHP.eB-Null | Inquiry |
| AAV00118Z | AAV PHP.S-Null | Inquiry |
| AAV00295Z | AAV PHP.B-GFP | Inquiry |
| AAV00296Z | AAV PHP.B-mCherry | Inquiry |
| AAV00297Z | AAV PHP.B-Cre | Inquiry |
| AAV00298Z | AAV PHP.B-Cre-GFP | Inquiry |
| AAV00299Z | AAV PHP.B-CAG-GFP | Inquiry |
| AAV00300Z | AAV PHP.B-CAG-mCherry | Inquiry |
| AAV00301Z | AAV PHP.B-Syn-GFP | Inquiry |
| AAV00302Z | AAV PHP.eB-CMV-GFP | Inquiry |
| AAV00303Z | scAAV PHP.eB-CMV-GFP | Inquiry |
| AAV00304Z | AAV PHP.eB-CMV-Cre | Inquiry |
| AAV00305Z | AAV PHP.eB-CAG-GFP | Inquiry |
| AAV00306Z | AAV PHP.eB-CAG-Cre | Inquiry |
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