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CpG oligodeoxynucleotides (CpG ODN) are key molecules in modern immunotherapy and vaccine development. By mimicking unmethylated CpG motifs from bacterial and viral DNA sequences, they activate Toll-like receptor 9 (TLR9) to trigger robust immune responses. They are widely used in vaccine adjuvants and immunotherapy, effectively initiating adaptive immune responses. In 2017, the first CpG ODN adjuvant-containing vaccine, Heplisav-B, was approved by the US FDA, marking a clinical breakthrough for CpG ODN technology. Currently, over 100 CpG adjuvant-containing vaccines are in clinical trials, covering multiple fields including cancer immunotherapy and infectious disease prevention, demonstrating tremendous application potential.
Figure 1. Mechanism of Action of CpG ODNs.
Creative Biogene possesses advanced automated oligonucleotide synthesis platforms and mature purification and analysis workflows, enabling us to provide high-quality, high-purity, high-activity CpG ODN synthesis services to global research and biopharmaceutical customers. We offer comprehensive support from sequence design, structural modification, and functional validation to final delivery, suitable for different stages from basic research to preclinical development.
We can synthesize the following types of CpG ODN molecules with deep customization support for structure and function:
CpG-A ODNs (Type A/D-type)
Example: ODN 2216 (5'-ggGGGACGATCGTCgg999-3')
These ODNs feature a central palindromic CpG motif flanked by poly-G tails, with partial phosphorothioate modification. They strongly activate plasmacytoid dendritic cells (pDCs) and induce high levels of IFN-α, but show limited stimulation of B cells. CpG-A is commonly used in tumor immunotherapy and antiviral vaccine research.
CpG-B ODNs (Type B/K-type)
Example: ODN 2006 (5'-tcgtcgttttgtcgttttgtcgtt-3')
Fully phosphorothioated with a linear structure, CpG-B primarily activates B cells and promotes IgM secretion, but has minimal effect on pDCs. It is ideal for use as a vaccine adjuvant and in antibody production applications.
CpG-C ODNs
Example: ODN 2395 (5'-tcgtcgttttcggcgcgcgccg-3')
Combining full phosphorothioate modification with a palindromic sequence, CpG-C can form dimers and activate both pDCs and B cells. It offers the combined benefits of A- and B-type ODNs, making it suitable for broad immune stimulation, autoimmune models, and vaccine development.
We support any length (10-50 nt), different modifications (phosphorylation, fluorescence, biotin, biodegradable modifications), and ligand co-delivery schemes.
Multi-platform synthesis capability
Solid-phase synthesis technology, adaptable for small-scale and GMP-grade production
High modification flexibility
Supports conventional and special backbone modifications, fluorescent probes, and branched structure construction
High batch-to-batch consistency
Achieves 100% batch consistency, suitable for long-term projects and preclinical applications
Activity validation support
Provides immune stimulation function testing and TLR9-dependent analysis services
We provide comprehensive customized services from design to delivery:
1Sequence Design Optimization: Optimize CpG sequence configuration based on target cells, species, and immune pathways
2Synthesis and Modification: Use automated DNA synthesizers for step-by-step base construction with precise structural control
3Purification and Identification: Complete testing with high-performance liquid chromatography (HPLC), MALDI-TOF, and UV spectrophotometry
4Functional Validation (Optional): Immune cell stimulation, IFN-α/IL-6 release experiments
5Product Delivery: Provide lyophilized powder, solution, or plate formats according to customer requirements
| Item | Standard Description |
| Nucleic Acid Purity | ≥95%, verified by HPLC or CE |
| Molecular Weight Confirmation | MALDI-TOF or ESI-MS |
| Concentration Calibration | A260 method or fluorescence quantification |
| Endotoxin Content | <5 EU/mg (LAL method) |
| Sterile Processing | RNase-free, sterile versions available |
| Biological Activity Verification | TLR9 agonist effect detection available (optional) |
We understand that every CpG ODN project has its unique characteristics. Whether you are in the basic research stage or advancing clinical translation, Creative Biogene can provide tailored, reliable, and translatable CpG ODN solutions to meet your needs.
Q: Which type of CpG ODN (A/B/C type) should I choose?
A: The choice depends mainly on your research objectives. CpG-A is suitable for inducing interferons and studying dendritic cell functions; CpG-B is appropriate for B cell activation and vaccine adjuvant development; CpG-C combines A/B advantages and is ideal for constructing comprehensive immune response models. If you're unsure, we can provide free consultation and sequence recommendations.
Q: Can you provide a GMP-standard CpG ODN?
A: Yes, we support synthesis processes from research-grade to GMP-level, including production, quality control, and documentation output compliant with ICH-Q7, FDA, and NMPA requirements, suitable for preclinical applications and IND submission materials.
Q: Can I order CpG molecules with specific modifications?
A: Absolutely. We support various terminal modifications, internal modifications, and conjugation methods, including FAM, Cy5, biotin, lipid chains, nucleic acid ligands, etc., for tracking, functional validation, or delivery system construction.
Q: Do you provide immune function experiments? Can you outsource part of the validation?
A: We can provide in vitro functional experiment support (such as PBMC stimulation, IFN-α release assays, etc.) and can also assist in outsourcing in vivo experiments or connecting with third-party CRO resources to help customers advance project validation more quickly.
Q: What is the typical product delivery cycle? Can it be expedited?
A: The standard synthesis cycle is 7-12 working days; special modifications or large-scale synthesis may take slightly longer. We offer expedited services (delivery within 5 working days at a minimum). Please contact us to evaluate specific timelines.
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