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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | DLD-1 is a specific type of human colorectal cancer cell line. It was derived from a tumor in the colon of a 65-year-old male patient. DLD-1 reacts positively to p53 protein and various oncogenes (such as C-Myc, KRAS, HRAS and NRAS, etc.). Some studies have pointed out that DLD-1 cells have strong crawling and invasion abilities, so they have high migration and invasion abilities. |
| Tissue | Large intestine; Colon |
| Disease | Adenocarcinoma; Colorectal; Dukes' type C |
| Morphology | Epithelial |
| Gender | Male |
| Age | 65 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | The DLD-1 cell line has been used to express shRNA oligonucleotides directed against Coronin 2A (CRN5) and to study the role of CRN5 in tumor cell migration. It was also used to determine the efficiency of antibody-conjugated short interfering RNA (siRNA) for in vivo mRNA knockdown. |
| Shipped in | dry ice |
| Storage Temperature | −196°C |
| Additional Info | DLD-1 cells have been used in the study of polar solvents on cell characteristics.The four cell lines: DLD-1, HCT-15, HCT-8 and HRT-18 have been shown to have a common genetic origin. |
| Characteristics | |
| Viruses | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Mycoplasma Test | Negative |
| Karyotype | 2n = 46, pseudodiploid |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Cells that are difficult to separate can be placed at 37°C to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37°C. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Doubling Time | ca. 48 hours |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:3 to 1:10 is recommended. |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | Frozen with 70% medium, 20% FBS, 10% DMSO |
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