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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | Capan-2 is a human pancreatic adenocarcinoma cell line established in 1975, derived from a 56-year-old Caucasian male patient. This cell line was isolated from a primary pancreatic tumor. Capan-2 cells exhibit an epithelial morphology and grow as an adherent monolayer. As a well-characterized cellular model, this cell line is frequently utilized in research related to pancreatic ductal adenocarcinoma (PDAC). Unlike the more aggressive Capan-1 cell line, Capan-2 cells display relatively low morphological heterogeneity and retain a high degree of ductal differentiation characteristics. This cell line is commonly employed to investigate the role of KRAS gene mutations in tumor initiation and progression, the expression patterns of blood group antigens, and the efficacy evaluation of therapeutic agents targeting pancreatic malignancies. |
| Tissue | Pancreas |
| Disease | Adenocarcinoma; Pancreatic Cancer |
| Morphology | Epithelial |
| Gender | Male |
| Age | 56 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Pancreatic ductal adenocarcinoma (PDAC) biology research 2. Study of KRAS-driven oncogenic signaling pathways 3. Investigation of pancreatic cell differentiation and mucin production 4. Drug screening and cytotoxicity assays for pancreatic cancer 5. Development of human tumor xenograft models in nude mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Hypertriploid; modal number = 74; range = 64 to 78 |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Genetic Profile | KRAS mutation; TP53 wild-type; SMAD4/DPC4 positive |
| Antigen Expression | Blood group Y; HLA-A2, B12, B17 |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with PBS (without Ca2+/Mg2+) to remove all traces of serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin - 0.53 mM EDTA solution and observe under an inverted microscope until the cell layer is dispersed (usually 5 to 15 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to obtain a single-cell suspension. 5. Aliquot cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:2 to 1:4 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00747 | Luciferase Reporter Cell Line - Capan-2 | Inquiry |
| CSC-RR0433 | GFP Stable Cell Line - Capan-2 | Inquiry |
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