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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | HCT-15 cells were derived from a colon adenocarcinoma in a 44-year-old Caucasian male. Developed in the early 1970s, this cell line has been widely used in the field of cancer research, particularly to explore the biology and treatment of colorectal cancer. HCT15 cells are known for their ability to form spheroids, or cell aggregates, which makes them a valuable tool for studying the interaction between cancer cells and the surrounding microenvironment. Genotypically, HCT-15 cells exhibit a hyperdiploid karyotype with multiple chromosomal aberrations, which are typical of many colorectal cancers. These include mutations in key oncogenes and tumor suppressor genes, such as mutations in the KRAS gene and deletions affecting the p53 pathway, which have been implicated in the pathogenesis and progression of colorectal cancer. These genetic properties make HCT-15 cells an important tool for studying the genetic and molecular mechanisms associated with cancer progression, metastasis, and resistance to therapies. |
| Tissue | Colorectal |
| Disease | Adenocarcinoma; Colorectal; Dukes' type C |
| Morphology | Epithelial |
| Gender | Male |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Drug testing and development 3. Genetic studies 4. Signal transduction research 5. Biomarker identification 6. Radiation research 7. Resistance mechanism studies |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The HCT-15 cell line was used to create the CDX (cell line derived xenograft) HCT-15 xenograft mouse model. The HCT-15 xenograft model has been used to evaluate anticancer therapies for colon cancer, including viral therapy, antibody therapy, and the Phellodendron amurense extract nexrutine (NX). |
| Characteristics | |
| Karyotype | This is a quasi-diploid human cell line with a mode number of 46 in 76% of cells (range = 41 to 47 for 50 metaphases). The polyploidy rate is 5.1%. The karyotype of this cell line is 46, XY, 8, 11, 17, t(8: 17)(p23: q21), inv(11)(p15.3Q13.1). The Y chromosome is slightly longer than N22 and has a large heterochromatic fluorescent distal q arm. |
| Antigen Expression | The cells are positive for keratin by immunoperoxidase staining. |
| Tumorigenic | Yes, in nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | The volumes used in this protocol are for 75 cm2 flasks. For culture vessels of other sizes, the amount of dissociation medium should be proportionally reduced or increased. 1. Remove and discard the medium. 2. Rinse the cell layer briefly with 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37°C to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette out the cells. 5. Add an appropriate aliquot of the cell suspension to a new culture vessel. 6. Incubate the culture at 37°C. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:2 to 1:10 is recommended. |
| Culture Medium | RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS) (heat inactivated). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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