Cat.No. : CSC-RR0433 Host Cell: Capan-2
| Cat. No. | CSC-RR0433 |
| Description | Capan-2/GFP is engineered to stably express GFP. It is a good ready-to-use cell model for tracing Capan-2 cells. |
| Host Cell | Capan-2 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The GFP Stable Cell Line - Capan-2 is engineered to stably express the green fluorescent protein (GFP), which serves as a marker for visualizing cellular structures and tracking protein dynamics in live cells. This cell line facilitates research by allowing real-time imaging of GFP-tagged proteins, enabling the study of their subcellular localization and movement. Researchers should follow strict protocols for cell culture, including maintaining optimal growth conditions and selecting appropriate media. Additionally, they must ensure proper handling of the imaging equipment, such as setting the correct exposure times and fluorescence filters, to capture high-quality images that accurately represent the protein's behavior within the cells.
A: The GFP Stable Cell Line - Capan-2, derived from a pancreatic cancer cell line, offers a unique platform for studying the molecular mechanisms that drive pancreatic cancer progression. By utilizing this cell line, researchers can investigate the behavior of GFP-tagged proteins that are implicated in cancer development and metastasis. The insights gained from these studies can lead to a better understanding of the signaling pathways and cellular processes that contribute to tumor growth and invasion. This knowledge can then be applied to the design of targeted therapies that specifically address these molecular mechanisms, potentially leading to more effective treatments for pancreatic cancer.
A: When employing the GFP Stable Cell Line - Capan-2 for validating gene editing technologies, it is crucial to consider factors such as the efficiency of the gene editing system, the specificity of the guide RNA, and the potential for off-target effects. The success of gene modification experiments is highly dependent on the precise delivery of the editing components into the cells and the subsequent repair of the targeted DNA sequence. Researchers must optimize the transfection conditions, validate the guide RNA design through bioinformatics tools, and use appropriate controls to assess the editing efficiency and specificity. Additionally, they should employ robust methods to detect and quantify the edited cells, such as flow cytometry or fluorescence microscopy, to ensure the reliability of the experimental outcomes.
A: The GFP Stable Cell Line - Capan-2 can be effectively integrated into high-throughput screening assays by labeling key proteins involved in cancer cell survival and proliferation with GFP. This allows for the rapid and quantitative assessment of the effects of various compounds on these cellular processes. Researchers can use automated imaging systems and image analysis software to screen large libraries of compounds for their ability to alter GFP fluorescence, which correlates with changes in protein levels or activity. By identifying compounds that modulate these processes, researchers can uncover potential anti-cancer agents and gain insights into the underlying molecular mechanisms.
A: The GFP Stable Cell Line - Capan-2 offers several advantages for studying the effects of environmental toxins on cellular stress responses. The stable expression of GFP allows for the direct visualization of changes in cellular morphology and stress responses without the need for additional labeling or fixation. This cell line can be used to assess the impact of toxins on cellular processes such as apoptosis, autophagy, and oxidative stress. By identifying changes in GFP fluorescence patterns or subcellular localization, researchers can develop biomarkers that indicate exposure to specific environmental toxins. These biomarkers can then be used for environmental monitoring and risk assessment, contributing to public health and safety initiatives.
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The GFP in the GFP Stable Cell Line - Capan-2 offers clear fluorescent tagging of cells, allowing for easy identification and tracking under a fluorescence microscope.
This cell line exhibits adherent growth, which simplifies the handling and downstream applications such as microscopy and molecular assays.
GFP Stable Cell Line - Capan-2 has stable integration of the GFP gene, ensuring consistent expression and reliable fluorescent signal across experiments.
The GFP expression allows for live-cell imaging of cellular dynamics, providing an advantage in monitoring the effects of drugs in real-time with the GFP Stable Cell Line - Capan-2.
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