GFP Stable Cell Line - Capan-2

Name: GFP Stable Cell Line - Capan-2
SKU: CSC-RR0433
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0433

Host Cell : Capan2 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RR0433
Description	Capan-2/GFP is engineered to stably express GFP. It is a good ready-to-use cell model for tracing Capan-2 cells.
Host Cell	Capan2
Host Cell Species	Homo sapiens (Human)
Reporter Type	Fluorescent protein
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Background

Applications

Q & A

Customer Reviews

The Capan-2 cell line is a human pancreatic cancer cell line that is used to study the biology of pancreatic cancer and for drug development. The stable expression of the GFP reporter gene in Capan-2 cells enables researchers to visualize cellular processes, such as cell growth, migration, and the response to potential therapeutic agents. GFP serves as a non-invasive marker that can be observed in living cells, providing real-time information on the behavior of pancreatic cancer cells.

The GFP stable cell line in Capan-2 cells is a valuable tool for studying the molecular mechanisms underlying pancreatic cancer development and for evaluating the efficacy of potential therapeutic agents. It allows for the tracking of cancer cells in vitro and in vivo, facilitating the understanding of disease progression and the development of targeted cancer therapies.

The Capan-2 GFP Stable Cell Line is a human pancreatic cancer cell line that expresses GFP. This cell line is valuable for studying the characteristics of pancreatic cancer and for the development of novel therapeutic strategies.

(1)Cancer Cell Biology: The Capan-2 GFP cells can be used to investigate the biology of pancreatic cancer cells, including their growth, survival, and response to therapeutic agents. The GFP expression allows for easy visualization of cellular processes.

(2)Drug Discovery: This cell line can be employed in drug screening assays to identify compounds that target pancreatic cancer cells. The GFP signal can be used to assess changes in cell viability and proliferation in response to treatment.

(3)In Vivo Tumor Models: The Capan-2 GFP cell line is suitable for the establishment of in vivo tumor models. The GFP expression facilitates the tracking of tumor growth and metastasis in animal models, providing a valuable tool for preclinical studies.

Customer Q&As

How does the GFP Stable Cell Line - Capan-2 facilitate the study of protein localization and dynamics within the cellular environment, and what are the key experimental protocols that researchers should follow to ensure accurate observations?

A: The GFP Stable Cell Line - Capan-2 is engineered to stably express the green fluorescent protein (GFP), which serves as a marker for visualizing cellular structures and tracking protein dynamics in live cells. This cell line facilitates research by allowing real-time imaging of GFP-tagged proteins, enabling the study of their subcellular localization and movement. Researchers should follow strict protocols for cell culture, including maintaining optimal growth conditions and selecting appropriate media. Additionally, they must ensure proper handling of the imaging equipment, such as setting the correct exposure times and fluorescence filters, to capture high-quality images that accurately represent the protein's behavior within the cells.

In the context of cancer research, what unique insights can the GFP Stable Cell Line - Capan-2 provide into the molecular mechanisms underlying pancreatic cancer progression, and how might these insights inform the development of targeted therapies?

A: The GFP Stable Cell Line - Capan-2, derived from a pancreatic cancer cell line, offers a unique platform for studying the molecular mechanisms that drive pancreatic cancer progression. By utilizing this cell line, researchers can investigate the behavior of GFP-tagged proteins that are implicated in cancer development and metastasis. The insights gained from these studies can lead to a better understanding of the signaling pathways and cellular processes that contribute to tumor growth and invasion. This knowledge can then be applied to the design of targeted therapies that specifically address these molecular mechanisms, potentially leading to more effective treatments for pancreatic cancer.

What are the critical factors to consider when using the GFP Stable Cell Line - Capan-2 for the validation of novel gene editing technologies, such as CRISPR/Cas9, and how can these factors influence the success of gene modification experiments?

A: When employing the GFP Stable Cell Line - Capan-2 for validating gene editing technologies, it is crucial to consider factors such as the efficiency of the gene editing system, the specificity of the guide RNA, and the potential for off-target effects. The success of gene modification experiments is highly dependent on the precise delivery of the editing components into the cells and the subsequent repair of the targeted DNA sequence. Researchers must optimize the transfection conditions, validate the guide RNA design through bioinformatics tools, and use appropriate controls to assess the editing efficiency and specificity. Additionally, they should employ robust methods to detect and quantify the edited cells, such as flow cytometry or fluorescence microscopy, to ensure the reliability of the experimental outcomes.

How can the GFP Stable Cell Line - Capan-2 be integrated into high-throughput screening assays to identify compounds that modulate cellular processes related to cancer cell survival and proliferation?

A: The GFP Stable Cell Line - Capan-2 can be effectively integrated into high-throughput screening assays by labeling key proteins involved in cancer cell survival and proliferation with GFP. This allows for the rapid and quantitative assessment of the effects of various compounds on these cellular processes. Researchers can use automated imaging systems and image analysis software to screen large libraries of compounds for their ability to alter GFP fluorescence, which correlates with changes in protein levels or activity. By identifying compounds that modulate these processes, researchers can uncover potential anti-cancer agents and gain insights into the underlying molecular mechanisms.

What are the advantages of using the GFP Stable Cell Line - Capan-2 for studying the effects of environmental toxins on cellular stress responses, and how can these studies contribute to the development of biomarkers for environmental monitoring?

A: The GFP Stable Cell Line - Capan-2 offers several advantages for studying the effects of environmental toxins on cellular stress responses. The stable expression of GFP allows for the direct visualization of changes in cellular morphology and stress responses without the need for additional labeling or fixation. This cell line can be used to assess the impact of toxins on cellular processes such as apoptosis, autophagy, and oxidative stress. By identifying changes in GFP fluorescence patterns or subcellular localization, researchers can develop biomarkers that indicate exposure to specific environmental toxins. These biomarkers can then be used for environmental monitoring and risk assessment, contributing to public health and safety initiatives.

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Customer Reviews

Clear Fluorescent Tagging

The GFP in the GFP Stable Cell Line - Capan-2 offers clear fluorescent tagging of cells, allowing for easy identification and tracking under a fluorescence microscope.

French

2023-04-05

Adherent Growth Characteristic

This cell line exhibits adherent growth, which simplifies the handling and downstream applications such as microscopy and molecular assays.

French

2022-09-15

Stable GFP Integration

GFP Stable Cell Line - Capan-2 has stable integration of the GFP gene, ensuring consistent expression and reliable fluorescent signal across experiments.

United Kingdom

2021-09-18

Live-Cell Imaging Capability

The GFP expression allows for live-cell imaging of cellular dynamics, providing an advantage in monitoring the effects of drugs in real-time with the GFP Stable Cell Line - Capan-2.

Canada

2023-02-27

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GFP Stable Cell Line - Capan-2

Cell Line Information

Cell Culture Information

Safety and Packaging

Background

Applications

Q & A

Customer Reviews

How does the GFP Stable Cell Line - Capan-2 facilitate the study of protein localization and dynamics within the cellular environment, and what are the key experimental protocols that researchers should follow to ensure accurate observations?

In the context of cancer research, what unique insights can the GFP Stable Cell Line - Capan-2 provide into the molecular mechanisms underlying pancreatic cancer progression, and how might these insights inform the development of targeted therapies?

What are the critical factors to consider when using the GFP Stable Cell Line - Capan-2 for the validation of novel gene editing technologies, such as CRISPR/Cas9, and how can these factors influence the success of gene modification experiments?

How can the GFP Stable Cell Line - Capan-2 be integrated into high-throughput screening assays to identify compounds that modulate cellular processes related to cancer cell survival and proliferation?

What are the advantages of using the GFP Stable Cell Line - Capan-2 for studying the effects of environmental toxins on cellular stress responses, and how can these studies contribute to the development of biomarkers for environmental monitoring?

Clear Fluorescent Tagging

Adherent Growth Characteristic

Stable GFP Integration

Live-Cell Imaging Capability

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