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Cat.No. : CSC-RO0244 Host Cell: HT1080
| Cat. No. | CSC-RO0244 |
| Description | This cell line is engineered to stably express human TNFRSF18 gene. |
| Gene | TNFRSF18 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HT1080 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Check if the culture medium components are suitable for this cell line, regularly replace with fresh medium, and consider reducing the frequency of passaging to maintain lower passage numbers.
A: Ensure uniform experimental conditions, including cell density, concentration and duration of treatment agents, use standardized apoptosis detection methods, and regularly validate the phenotype and function of the cell line.
A: Optimize the oxygen concentration in the incubator, possibly using a hypoxia chamber or adjusting the gas composition in the incubator to provide a suitable low-oxygen environment.
A: Test different transfection reagents and methods, such as viral vectors, electroporation, or liposomes, and optimize culture conditions before and after transfection.
A: Optimize the construction of the expression vector, including the selection of promoters and enhancers, adjust culture conditions to optimize protein expression, and if necessary, use gene amplification techniques.
A: Optimize the staining protocol, including the type and concentration of antibodies, incubation time, and treatment methods of flow cytometry samples, to ensure more stable and consistent detection results.
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