Human TNFRSF18 Stable Cell Line-HT1080

Name: Human TNFRSF18 Stable Cell Line-HT1080
SKU: CSC-RO0244
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0244

Host Cell : HT1080 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0244
Description	This cell line is engineered to stably express human TNFRSF18 gene.
Target Gene	TNFRSF18
Gene Species	Homo sapiens (Human)
Host Cell	HT1080
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFRSF18 tumor necrosis factor receptor superfamily, member 18 [ Homo sapiens ]
Gene Symbol	TNFRSF18
Synonyms	AITR; GITR; CD357; GITR-D
Gene Description	tumor necrosis factor receptor superfamily, member 18
GeneID	8784
Uni ProtID	Q9Y5U5
mRNA Refseq	NM_148901.1
Protein Refseq	NP_683699.1
Chromosome Location	1p36.3
Function	tumor necrosis factor-activated receptor activity;
Pathway	Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem;
MIM	603905

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How to resolve the slowdown in growth rate of Human TNFRSF18 Stable Cell Line-HT1080 after long-term culture?

A: Check if the culture medium components are suitable for this cell line, regularly replace with fresh medium, and consider reducing the frequency of passaging to maintain lower passage numbers.

How to improve the consistency of apoptosis experiments in Human TNFRSF18 Stable Cell Line-HT1080?

A: Ensure uniform experimental conditions, including cell density, concentration and duration of treatment agents, use standardized apoptosis detection methods, and regularly validate the phenotype and function of the cell line.

How to improve the growth of Human TNFRSF18 Stable Cell Line-HT1080 under hypoxic conditions?

A: Optimize the oxygen concentration in the incubator, possibly using a hypoxia chamber or adjusting the gas composition in the incubator to provide a suitable low-oxygen environment.

How to enhance transfection efficiency in Human TNFRSF18 Stable Cell Line-HT1080?

A: Test different transfection reagents and methods, such as viral vectors, electroporation, or liposomes, and optimize culture conditions before and after transfection.

How to deal with unstable protein overexpression in Human TNFRSF18 Stable Cell Line-HT1080?

A: Optimize the construction of the expression vector, including the selection of promoters and enhancers, adjust culture conditions to optimize protein expression, and if necessary, use gene amplification techniques.

How to improve the stability of surface marker detection in flow cytometry analysis of Human TNFRSF18 Stable Cell Line-HT1080?

A: Optimize the staining protocol, including the type and concentration of antibodies, incubation time, and treatment methods of flow cytometry samples, to ensure more stable and consistent detection results.

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