Luc Reporter Cell Line-HT1080 is a polyclonal population which is constructed by lentivirus transduction, followed by stable cell selection. This cell line stably expresses luciferase gene and puromycin resistance gene driven by spleen focus-forming virus (SFFV) promoter. It is an ideal positive control for use in bioluminescence assays. In addition, HT1080 cells form tumors post implantation into immunocompromised mice. The in vivo growth of these tumors can be monitored using bioluminescent imaging.