Cat.No. : CSC-RR0274 Host Cell: HT1080
| Cat. No. | CSC-RR0274 |
| Description | Luc Reporter Cell Line-HT1080 is engineered to stably overexpress luciferase reporter gene. It is an ideal positive control for use in bioluminescence assays. In addition, HT1080 cells form tumors post implantation into immunocompromised mice. The in vivo growth of these tumors can be monitored using bioluminescent imaging. |
| Gene | Luciferase |
| Host Cell | HT1080 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Luc Reporter gene is a fusion gene that combines a luciferase gene with a promoter region, allowing for the measurement of gene expression levels through bioluminescence.
A: Luciferase enzyme produced by the Luc Reporter gene catalyzes a reaction that results in the production of light, which can be measured to quantify the expression of the linked promoter region.
A: Luc Reporter gene provides a sensitive and non-invasive method for monitoring gene expression in real-time, making it useful for various molecular and cellular biology research.
A: Yes, Luc Reporter gene can be used in vivo, allowing for the visualization and quantification of gene expression in living organisms, which is particularly useful in gene therapy and disease modeling studies.
A: While Luc Reporter gene is a powerful tool, it requires specialized equipment for light detection and may be sensitive to environmental factors, such as temperature and oxygen levels.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Luc Reporter Cell Line - HT1080 shows high luciferase activity, providing me with reliable data for my research.
This cell line is safe to use and consistently produces accurate results, giving me confidence in its performance.
The Luc Reporter Cell Line - HT1080 is easy to use and requires minimal maintenance, providing me with convenience for my research.
This product is good value for money, providing efficient and reliable results without breaking the bank.
use The Luc Reporter Cell Line-HT1080 can be used for a variety of research applications and is a great tool for my research.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
