Cat.No. : CSC-RO0243 Host Cell: HT1080
| Cat. No. | CSC-RO0243 |
| Description | This cell line is engineered to stably express human TNFRSF4 gene. |
| Gene | TNFRSF4 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HT1080 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human TNFRSF4 Stable Cell Line-HT1080 has been engineered to overexpress TNFRSF4, so its expression is considerably higher than in native HT1080 cells and most other standard cell lines used in immunological studies, unless they've also been modified for TNFRSF4 expression.
A: Yes, the Human TNFRSF4 Stable Cell Line-HT1080 is an ideal model for such studies. Its overexpression of TNFRSF4 allows for a more discernible study of interactions between OX40L and TNFRSF4 compared to non-engineered cells.
A: While standard culture conditions suffice for most purposes, it's advisable to use a selective antibiotic to maintain the integration and expression of the TNFRSF4 gene. Always refer to the cell line's product guide for specific recommendations.
A: While the fundamental signaling pathways triggered by TNFRSF4 engagement should be conserved, the amplitude and kinetics of the response might differ in the overexpressing Human TNFRSF4 Stable Cell Line-HT1080 compared to primary T cells. It's crucial to consider these potential differences when interpreting experimental results.
A: The Human TNFRSF4 Stable Cell Line-HT1080 provides a unique system to evaluate the interplay of TNFRSF4-mediated signaling with other co-stimulatory and co-inhibitory pathways. While this cell line might exhibit pronounced responses due to TNFRSF4 overexpression, it's essential to validate findings in primary immune cell systems to ensure physiological relevance.
A: Yes, the overexpression of TNFRSF4 in this cell line makes it suitable for high-throughput screens. Researchers should ensure consistent TNFRSF4 expression across batches and consider potential off-target effects, especially if the screen involves genetic perturbations.
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