Human TNFRSF4 Stable Cell Line-HT1080

Name: Human TNFRSF4 Stable Cell Line-HT1080
SKU: CSC-RO0243
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0243

Host Cell : HT1080 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0243
Description	This cell line is engineered to stably express human TNFRSF4 gene.
Target Gene	TNFRSF4
Gene Species	Homo sapiens (Human)
Host Cell	HT1080
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFRSF4 tumor necrosis factor receptor superfamily, member 4 [ Homo sapiens ]
Gene Symbol	TNFRSF4
Synonyms	OX40; ACT35; CD134; TXGP1L
GeneID	7293
Uni ProtID	P43489
mRNA Refseq	NM_003327.3
Protein Refseq	NP_003318.1
Chromosome Location	1p36
Function	tumor necrosis factor-activated receptor activity;
Pathway	Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem;
MIM	600315

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

Human TNFRSF4 Stable Cell Line-HT1080

A: The Human TNFRSF4 Stable Cell Line-HT1080 has been engineered to overexpress TNFRSF4, so its expression is considerably higher than in native HT1080 cells and most other standard cell lines used in immunological studies, unless they've also been modified for TNFRSF4 expression.

Can the Human TNFRSF4 Stable Cell Line-HT1080 be utilized as a suitable model for studying ligand-receptor interactions, specifically focusing on OX40L binding to TNFRSF4?

A: Yes, the Human TNFRSF4 Stable Cell Line-HT1080 is an ideal model for such studies. Its overexpression of TNFRSF4 allows for a more discernible study of interactions between OX40L and TNFRSF4 compared to non-engineered cells.

When culturing the Human TNFRSF4 Stable Cell Line-HT1080, are there any special media additives or growth conditions that enhance the stability of TNFRSF4 expression?

A: While standard culture conditions suffice for most purposes, it's advisable to use a selective antibiotic to maintain the integration and expression of the TNFRSF4 gene. Always refer to the cell line's product guide for specific recommendations.

How does the signaling cascade activated by TNFRSF4 in the Human TNFRSF4 Stable Cell Line-HT1080 differ from that in primary T cells with endogenous TNFRSF4 expression?

A: While the fundamental signaling pathways triggered by TNFRSF4 engagement should be conserved, the amplitude and kinetics of the response might differ in the overexpressing Human TNFRSF4 Stable Cell Line-HT1080 compared to primary T cells. It's crucial to consider these potential differences when interpreting experimental results.

Given the role of TNFRSF4 in T cell co-stimulation, how does the Human TNFRSF4 Stable Cell Line-HT1080 respond to various immune checkpoint inhibitors in comparison to other model systems?

A: The Human TNFRSF4 Stable Cell Line-HT1080 provides a unique system to evaluate the interplay of TNFRSF4-mediated signaling with other co-stimulatory and co-inhibitory pathways. While this cell line might exhibit pronounced responses due to TNFRSF4 overexpression, it's essential to validate findings in primary immune cell systems to ensure physiological relevance.

Can the Human TNFRSF4 Stable Cell Line-HT1080 be leveraged in high-throughput screens to identify potential modulators of TNFRSF4-mediated signaling, and if so, what are the key considerations?

A: Yes, the overexpression of TNFRSF4 in this cell line makes it suitable for high-throughput screens. Researchers should ensure consistent TNFRSF4 expression across batches and consider potential off-target effects, especially if the screen involves genetic perturbations.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *

Name

Country *

Email *

Related Products

Cat.No.	Product Name	Price
CSC-RO0002	Human CD276 Stable Cell Line-CHO-K1	Inquiry
CSC-RO0011	Human CD274 Stable Cell Line-CHO-K1	Inquiry
CSC-RO0012	Human CD274 Stable Cell Line-HEK293T	Inquiry
CSC-RO0075	Human VTCN1 Stable Cell Line-HEK293T	Inquiry
CSC-RO0046	Human TNFRSF4 Stable Cell Line-CHO-K1	Inquiry
CSC-RO0112	Human CD274 Stable Cell Line-HEK293T	Inquiry
CSC-RO0113	Human PDCD1LG2 Stable Cell Line-HEK293T	Inquiry
CSC-RO0013	Human HAVCR2 Stable Cell Line-CHO-K1	Inquiry
CSC-RO0080	Human CD28 Stable Cell Line-HEK293T	Inquiry
CSC-RO0100	Human CTLA4 Stable Cell Line-HEK293T	Inquiry

Human TNFRSF4 Stable Cell Line-HT1080

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

Human TNFRSF4 Stable Cell Line-HT1080

Can the Human TNFRSF4 Stable Cell Line-HT1080 be utilized as a suitable model for studying ligand-receptor interactions, specifically focusing on OX40L binding to TNFRSF4?

When culturing the Human TNFRSF4 Stable Cell Line-HT1080, are there any special media additives or growth conditions that enhance the stability of TNFRSF4 expression?

How does the signaling cascade activated by TNFRSF4 in the Human TNFRSF4 Stable Cell Line-HT1080 differ from that in primary T cells with endogenous TNFRSF4 expression?

Given the role of TNFRSF4 in T cell co-stimulation, how does the Human TNFRSF4 Stable Cell Line-HT1080 respond to various immune checkpoint inhibitors in comparison to other model systems?

Can the Human TNFRSF4 Stable Cell Line-HT1080 be leveraged in high-throughput screens to identify potential modulators of TNFRSF4-mediated signaling, and if so, what are the key considerations?

Related Products

Related Services