GFP Reporter Cell Line-HT1080

Human cancers frequently hijack signaling pathways crucial for normal tissue growth and organ development. Using intravital imaging, the researchers investigated the dynamics of Wnt signaling in tumor metastasis using the GFP Reporter Cell Line-HT1080. They found that different β-catenin/TCF-dependent and ATF2-dependent signaling activities were involved in cancer cell invasion, intravasation, and formation of metastatic lesion formation in chick embryo hosts. Cancer cells that had low canonical Wnt activity were more likely to invade and intravasate, while those that had reduced ATF2-dependent activity were less invasive. These findings were validated by concurrent use of a double-reporter cell line, highlighting the distinct roles of Wnt signaling branches in primary tumors and metastatic lesions, which are important for targeted therapeutic strategies in metastasis management.

Figure 1. The researchers generated HT1080-ATF2-GFP cells by transducing HT1080-tdTomato cells with an ATF2-GFP vector (pCLX CHOP-dGFP). This cell line serves to visualize ATF2 localization and dynamics in live cells. The method involves transduction with the GFP-tagged ATF2 construct, enabling real-time monitoring of ATF2 protein behavior under various conditions, and aiding in studies related to cellular stress response and gene regulation. (Stoletov K, et al., 2023)