Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RR0272
Host Cell : HT1080 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR0272 |
| Description | Luc/GFP Reporter Cell Line-HT1080 is engineered to stably co-express GFP and luciferase reporter genes. It is an ideal positive control for use in bioluminescence and fluorescence assays. In addition, HT1080 cells form tumors post implantation into immunocompromised mice. The in vivo growth of these tumors can be monitored using bioluminescent imaging. In vivo fluorescent imaging is not recommended due to high background autofluorescence. |
| Target Gene | Luciferase/GFP |
| Host Cell | HT1080 |
| Host Cell Species | Homo sapiens (Human) |
| Reporter Type | Fluorescent protein |
| Applications |
1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The Luc/GFP Reporter Cell Line-HT1080 product has demonstrated its superiority in many aspects. Firstly, the expressed cell line can provide both bioluminescence and green fluorescent protein reports, allowing users to simultaneously detect two different biological processes in a single cell line. This dual reporting mechanism greatly improves the efficiency of experiments and facilitates data interpretation.
The background fluorescence of the cell line expressed in the Luc/GFP Reporter Cell Line-HT1080 product is very low, which makes the signal clearer and reduces the possibility of misjudgment.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.