Cat.No. : CSC-RR0503 Host Cell: HL60
| Cat. No. | CSC-RR0503 |
| Description | This cell line is engineered to stably overexpress EGFP reporter gene. HL-60 cell line is a human leukemia cell line that has been widely used in laboratory research. This cell line is a useful tool for fluorescent tracking of HL60 cells. |
| Gene | GFP |
| Host Cell | HL60 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Prior studies have shown aberrant and abundant expression of GINS2 in various malignant solid tumors, including breast cancer, melanoma, and hepatic carcinoma. Researchers investigated the role and molecular mechanism of GINS2 in acute promyelocytic leukemia (APL) using the GFP Reporter Cell Line - HL60. They successfully synthesized interference plasmids targeting GINS2 and constructed recombinant adenovirus vectors to modulate GINS2 expression. Transfection of siRNA into HL60 cells resulted in downregulation of GINS2 mRNA and protein levels, inhibiting cell growth and altering the expression of apoptotic markers. Further analysis revealed inhibited DNA replication and cell cycle arrest at G2/M phase. Conversely, overexpression of GINS2 promoted HL-60 cell proliferation. This study highlights GINS2 as a potential target for leukemia gene therapy.
Figure 1. Researchers assess GFP expression levels in HL60 cells using the GFP Reporter Cell Line. This allows for efficient detection and quantification of gene expression changes, aiding in cellular imaging studies. (Zhang, X., et al., 2013)
A: The GFP Reporter Cell Line in HL60 cells serves as an excellent tool for studying gene expression dynamics and cellular behavior in leukemia research. HL60 cells are widely used as a model for acute myeloid leukemia (AML), offering a relevant background for investigating leukemia biology. The GFP reporter allows for visualization and tracking of leukemia cells, facilitating studies on differentiation, proliferation, and drug response.
A: The reliability and reproducibility of GFP expression in the HL60 reporter cell line are maintained through robust experimental protocols. Stable transfection methods are employed to introduce the GFP reporter gene into HL60 cells, followed by clonal selection to isolate cell lines with stable and consistent GFP expression levels. Regular validation and monitoring of GFP fluorescence ensure reliable data generation in experiments.
A: The GFP Reporter Cell Line in HL60 cells can be utilized for in vivo studies or preclinical models of leukemia. The GFP fluorescence allows for non-invasive tracking of leukemia cells in live animals, enabling researchers to monitor disease progression, metastasis, and response to treatment in real-time. This feature enhances the translational potential of studies using the HL60 reporter cell line.
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Consistent performance! The GFP Reporter Cell Line consistently provides robust signals, ensuring reproducible results and accurate interpretation of experimental outcomes. From studying leukemic cell behavior to evaluating treatment efficacy, this cell line serves as an invaluable resource for unraveling the complexities of leukemia biology and developing novel therapeutic strategies.
Streamlined experimentation! Its stable GFP expression simplifies experimental workflows, allowing for efficient data collection and analysis in leukemia research.
The GFP Reporter Cell Line in HL60 cells offers bright and clear GFP expression, enabling precise visualization and tracking of cellular processes in my leukemia research. This cell line facilitates detailed investigation of cell differentiation, proliferation, and response to therapy, advancing our understanding of leukemia biology.
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