Cat.No. : CSC-RR0464 Host Cell: HL60
| Cat. No. | CSC-RR0464 |
| Description | HL60-GFP/Luc is a cell line that is engineered to stably express GFP and luciferase. It is an ideal positive control for use in both fluorescence and bioluminescence assays. |
| Gene | GFP/Luc |
| Host Cell | HL60 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Acute myeloid leukemia (AML) is a hematologic malignancy characterized by the rapid growth of abnormal white blood cells. Cytarabine (AraC) is a cornerstone in AML treatment, but resistance remains a significant challenge. The researchers utilized the HL60-Luc cell line to study the combination of the CHK1 inhibitor GDC-0575 and AraC in overcoming this resistance. By introducing stable luciferase-expressing AML cell lines, they employed bioluminescence imaging (BLI) to monitor treatment response in vivo, showing that the combination therapy significantly reduced leukemic burden without affecting normal hematopoietic stem cells.
Figure 1. Using the U937-Luc and HL60-Luc cell lines, the researchers monitored cell metabolic activity, apoptosis, and tumor progression through bioluminescence imaging (BLI) and flow cytometry, showing enhanced treatment efficacy with the drug combination. (Di Tullio A, et al., 2017)
Our GFP/Luc Reporter Cell Line - HL60 provides a powerful tool for studying AML treatment strategies, including drug sensitivity and resistance mechanisms. With its ability to express GFP and luciferase, this cell line allows for real-time, non-invasive monitoring of cell viability and drug response in vivo.
A: The HL60 GFP/Luc reporter cell line has been strictly genetically engineered to stably express green fluorescent protein (GFP) and luciferase (Luciferase). During multiple passages and long-term culture, the expression levels of GFP and Luc remained stable and did not decrease significantly as the number of cell passages increased. This stability ensures the reliability and reproducibility of experimental data, making this cell line highly practical in various research applications. In addition, each batch of cell lines undergoes rigorous quality control and validation to ensure consistency and high quality.
A: When using the HL60 GFP/Luc reporter cell line to perform dual reporter experiments, special attention needs to be paid to the acquisition conditions of fluorescence signals and luminescence signals. First, select appropriate detection equipment and parameters to measure the signals of GFP and Luc respectively to avoid spectral overlap or interference. Second, ensure that the cells are grown under the same culture conditions, including temperature, humidity, and culture medium formulation, to ensure stable expression of fluorescent proteins and luciferase. In addition, when detecting luciferase activity, the substrate (such as D-luciferin) should be added accurately and the luminescence signal should be collected at appropriate time points to ensure the accuracy of the data.
A: To distinguish and quantify the GFP and Luc signals of the HL60 GFP/Luc reporter cell line in data analysis, the following methods can be adopted: First, use dedicated fluorescence and luminescence detection equipment, such as a fluorescence microscope and a fluorescence microplate reader, to collect GFP and Luc respectively. signal of. Secondly, perform quantitative analysis of fluorescence intensity and luminescence intensity, and use standard curve or internal standard method for data correction. For the GFP signal, the cell fluorescence intensity can be analyzed through image processing software, while for the Luc signal, a fluorescent microplate reader can be used to read the luminescence intensity and normalize the data to reduce differences between experiments. In addition, it is recommended to repeat the experiment multiple times to obtain the average value and standard deviation to improve the accuracy and credibility of the data.
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The stability of the cells gave me great experimental confidence. Through bioluminescence testing, I successfully measured the luciferase activity in the cells and clearly observed the expression of GFP under a fluorescence microscope. This is my first time doing a related experiment and I have a good experience. I hope my experiments will go well in the future.
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