Luciferase Reporter Cell Line - HL60

Name: Luciferase Reporter Cell Line - HL60
SKU: CSC-RR0510
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0510

Host Cell : HL60 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Documents

Cat. No.	CSC-RR0510
Description	This cell line is engineered to stably overexpress luciferase reporter gene. HL-60 cell line is a human leukemia cell line that has been widely used in laboratory research. This cell line is a useful tool for bioluminescent tracking of HL60 cells.
Target Gene	Luciferase
Host Cell	HL60
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

CSC-RR0510-Luciferase Reporter Cell Line - HL60-Datasheet

Quick Inquiry

Background

Case Study

Q & A

Customer Reviews

The Luc reporter system is a valuable tool in molecular biology research, enabling the study of gene expression and regulation in a sensitive, reliable, and versatile manner. Its applications span various fields, including basic research, drug discovery, and toxicology, making it an essential resource for advancing our understanding of biological processes and developing new therapeutic strategies.

HL60 Cell Line is a human promyelocytic leukaemia cell line that has been used extensively in leukaemia research. It was established from a patient with promyelocytic leukaemia and has become an important model for studying the pathogenesis and treatment of this disease. Some of the major advantages of using HL60 cells include rapid growth, ease of culture, and karyotypic stability. These cells also exhibit a high degree of tumourigenicity, making them suitable for preclinical studies of cancer treatment. In addition, HL60 cells express a wide range of cell surface antigens, making them suitable for immune-based studies. In conclusion, the HL60 Cell Line is an important tool for the understanding and treatment of promyelocytic leukaemia.

Antibodies are undoubtedly the most versatile class of biologics with a wide range of applications in basic research, disease diagnosis and therapy. Researchers have developed a luciferase-based assay, called the Malibu-Glo Assay, for optimizing antigen-binding structural domains with desirable properties and enabling sensitive detection of tumor antigens. The assay involves a recombinant immunoconjugate, called the Malibu-Glo reagent, which includes immunoglobulin or non-immunoglobulin antigen-binding structural domains linked to the marine luciferase gene. The researchers produced the reagent as a secreted protein in mammalian cells, maintaining the functional activity of the antigen-binding structural domain and luciferase. By using the Luciferase Reporter Cell Line - HL60 cell line, the researchers were able to directly utilize the crude supernatant containing the secreted Malibu-Glo reagent for cell surface antigen detection, avoiding cumbersome protein purification and labeling steps. This method further demonstrated its utility in selecting single-chain antibody fragments (scFvs) with desirable affinity properties for use in chimeric antigen receptors (CARs). In conclusion, the Malibu-Glo Assay is a rapid, simple, sensitive, specific and cost-effective antigen detection method with multiple applications in the fields of antibody engineering, antibody humanization and CAR-T cell therapy.

Figure 1 demonstrates the results of functional assessment of CD19-expressing cells and CD19-deficient control cells using different marine fluorokinase-generating Malibu-Glo reagents. (doi: 10.1038/s41598-020-59099-9) Figure 1. The researchers replaced Nluc with Gluc, Tluc16, and Mluc7 to test the scalability of the Nluc-based Malibu-Glo assay. The ability of these fusion constructs to express CD19 on the cell surface was assessed using HL60 (CD19-negative acute myeloid leukemia cell line) as a negative control. The results showed that all CD19-scFv1-luc Malibu-Glo reagents exhibited specific binding in CD19-positive cell lines, while there was no significant binding in HL60. This demonstrates that the Malibu-Glo assay system maintains enzymatic activity and specific binding regardless of which marine fluorokinase is used. (Natarajan V, et al., 2020)

Customer Q&As

What are the characteristics of HL60 Cell Line?

A: HL60 Cell Line exhibits characteristics typical of myeloid leukemia cells, such as the expression of myeloid cell markers and the production of reactive oxygen species. It is also sensitive to retinoic acid, which is used in the treatment of acute promyelocytic leukemia. The cells can be differentiated into mature granulocytes, which is a useful feature for studying leukemia cell differentiation.

Are there any limitations to using HL60 Cell Line?

A: While HL60 Cell Line is a valuable tool for research, there are some limitations to its use. It is a human cell line, and the results obtained from studying these cells may not be directly applicable to other types of leukemia. Additionally, the use of cell lines may not fully recapitulate the complex microenvironment and systemic factors that influence the development and treatment of human tumors. Therefore, findings should be validated using clinical samples or other models.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *