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Cat.No. : CSC-RT0091
Host Cell: HL60 Target Gene: KDM6B
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0091 |
| Description | HL60-KDM6B cell line is a stable cell line with a homozygous knockout of KDM6B |
| Target Gene | KDM6B |
| Host Cell | HL60 |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I cannot praise KDM6B Knockout Cell Line-HL60 enough. Its user-friendly design and robust functionality have made it an indispensable tool for studying gene function and unraveling molecular pathways.
United States
07/19/2022
KDM6B Knockout Cell Line-HL60 has revolutionized the way I approach gene editing experiments. Its consistent knockout efficiency and reproducibility have elevated the quality and reliability of my research.
France
11/03/2022
I have experimented with various gene knockout reagents, but none have matched the exceptional performance of KDM6B Knockout Cell Line-HL60. Its efficacy, versatility, and ease of use make it an invaluable asset in my scientific investigations.
United States
02/23/2023
KDM6B Knockout Cell Line-HL60 has truly surpassed my expectations. Its remarkable efficacy and ease of use have propelled my research to new heights.
United States
04/11/2023
I am genuinely impressed with the precision and reliability of KDM6B Knockout Cell Line-HL60. It has provided me with invaluable insights into gene function and has expanded the boundaries of my research.
United States
08/23/2020
Using KDM6B Knockout Cell Line-HL60 has been a competent assistants for my experiments. Its consistent and efficient knockout capabilities have allowed me to delve deeper into the complexities of genetic mechanisms.
United States
05/12/2021
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