Cat.No. : CSC-RR0248 Host Cell: A375
| Cat. No. | CSC-RR0248 |
| Description | GFP Reporter Cell Line-A375 is engineered to stably overexpress GFP reporter gene. It is an ideal positive control for use in fluorescence assays. In vivo fluorescent imaging is not recommended due to high background autofluorescence. |
| Gene | GFP |
| Host Cell | A375 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: A375 Cell Line is significant for drug testing as it can be used to screen the effectiveness of potential anti-melanoma drugs and to study the mechanisms of action of known therapies, aiding in the development of new treatments for melanoma.
A: A375 Cell Line is valuable for melanoma research due to its ability to grow rapidly in culture and its retention of characteristics similar to those of primary melanoma tumors.
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The GFP reporter cell line - A375 produces a strong and stable fluorescent signal, which is perfect for my research purposes.
This cell line proved to be safe and efficient in my experiments, providing reliable and accurate data.
The GFP Reporter Cell Line-A375 is easy to culture and handle, saving time and effort in my research activities.
The product performs well and is reasonably priced, making it a cost-effective solution for my research needs.
The GFP Reporter Cell Line-A375 consistently provides reliable and reproducible results, which is essential for my research.
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