Human CD274 Stable Cell Line-A375

Name: Human CD274 Stable Cell Line-A375
SKU: CSC-RO1026
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1026

Host Cell : A375 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO1026
Description	A375-CD274 cell line is engineered to stably overexpress human CD274.
Target Gene	CD274
Gene Species	Homo sapiens (Human)
Host Cell	A375
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD274 CD274 molecule [ Homo sapiens ]
Gene Symbol	CD274
Synonyms	CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296;
Gene ID	29126
Uni Prot ID	Q9NZQ7
m RNA Refseq	BC113734
Chromosome Location	9p24.1
Function	protein binding; protein tyrosine phosphatase activity; receptor activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; PD-1 signaling, organism-specific biosystem;
MIM	605402

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What type of cell is A375?

A: Malignant melanoma cells in humans.

What are the features of Human CD274 Stable Cell Line-A375?

A: The cells have a heavy granular feel. There are more black spots inside the cells and more granules outside the cells during passaging and culture. It is recommended to use high-quality serum and medium to culture the cells.

Are there any special considerations when culturing Human CD274 Stable Cell Line-A375?

A: The digestion or resuscitation process should be gentle, strictly control the digestion time and resuscitation operation standards, and it is recommended to observe the cells 24h after passaging or resuscitation, and it is recommended to change the liquid once to remove dead cells.

How to handle excessive cell debris during the cultivation process?

A: It is recommended to use suitable culture conditions; 2. If there are usually fewer fragments, you can skip the PBS resuspension step when passaging; if there are many fragments, it is recommended to wash with PBS several times.

How to remove the black particles outside the cells?

A: Most of the black particles outside the cells are cell fragments and cell metabolites. You can first suck away the medium and rinse with PBS to remove them, and then add new medium to continue culturing.

Why use A375 to express CD274?

A: A375 cells are ideal controls for NRAS mutant-A375 isogenic, suitable for 3D cell culture, high-throughput screening, toxicology, and immunooncology research, and are also suitable transfection hosts.

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