RFP Reporter Cell Line-A375 is a polyclonal population which is constructed by lentivirus transduction, followed by stable cell selection. This cell line stably expresses RFP driven by the spleen focus-forming virus (SFFV) promoter, and neomycin resistance gene driven by the PGK promoter. It is an ideal positive control for use in fluorescence assays. In addition, A375 cells form tumors post implantation into immunocompromised mice. The in vivo growth of these tumors can be monitored using optical imaging.