Luc Reporter Cell Line-A375 is a polyclonal population which is constructed by lentivirus transduction, followed by stable cell selection. This cell line stably expresses luciferase gene and puromycin resistance gene driven by spleen focus-forming virus (SFFV) promoter. It is an ideal positive control for use in bioluminescence assays. In addition, A375 cells form tumors post implantation into immunocompromised mice. The in vivo growth of these tumors can be monitored using bioluminescent imaging.