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Cat.No. : CSC-RO0867 Host Cell: A375
| Cat. No. | CSC-RO0867 |
| Description | This cell line is engineered to stably overexpress human MELTF (also named as CD228). |
| Gene | MELTF |
| Gene Species | Homo sapiens (Human) |
| Host Cell | A375 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The expression of the MELTF gene in A375 cells can provide insights into melanoma's molecular mechanisms. The observed strong cytoplasmic and membranous positivity in melanoma cells helps understand MELTF's role in cancer cell signaling and behavior.
A: High-throughput screening with A375 cells requires consideration of their chromosomal abnormalities and growth characteristics. The hypotriploid nature might influence drug responses, necessitating context-specific interpretation of screening results.
A: A375 cells can be used to study melanoma-immune interactions, especially in immuno-oncology research. However, in vitro limitations may not fully replicate in vivo complex immune interactions.
A: The MELTF gene in A375 cells shows a distinct expression pattern, especially in melanoma, compared to other cancers. This helps understand MELTF's unique role in different cancer contexts.
A: Ensuring reproducibility involves strict aseptic conditions, careful thawing, and handling. Avoiding excessive alkalinity and adhering to recommended culture conditions are crucial.
A: It's essential to use A375 cells strictly for laboratory research purposes, respecting the ethical guidelines that prohibit their use in human or animal therapeutic applications, consumption, or diagnostics.
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