Human MELTF Stable Cell Line - A375

Name: Human MELTF Stable Cell Line - A375
SKU: CSC-RO0867
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0867

Host Cell : A375 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0867
Description	This cell line is engineered to stably overexpress human MELTF (also named as CD228).
Target Gene	MELTF
Gene Species	Homo sapiens (Human)
Host Cell	A375
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

How does the MELTF gene expression in the Human MELTF Stable Cell Line - A375 influence melanoma research?

A: The expression of the MELTF gene in A375 cells can provide insights into melanoma's molecular mechanisms. The observed strong cytoplasmic and membranous positivity in melanoma cells helps understand MELTF's role in cancer cell signaling and behavior.

What are the challenges in using the Human MELTF Stable Cell Line - A375 for high-throughput drug screening?

A: High-throughput screening with A375 cells requires consideration of their chromosomal abnormalities and growth characteristics. The hypotriploid nature might influence drug responses, necessitating context-specific interpretation of screening results.

Can the Human MELTF Stable Cell Line - A375 be used for studying melanoma-immune system interactions, and what are the limitations?

A: A375 cells can be used to study melanoma-immune interactions, especially in immuno-oncology research. However, in vitro limitations may not fully replicate in vivo complex immune interactions.

How does the MELTF gene expression pattern in the Human MELTF Stable Cell Line - A375 compare to other cancer types?

A: The MELTF gene in A375 cells shows a distinct expression pattern, especially in melanoma, compared to other cancers. This helps understand MELTF's unique role in different cancer contexts.

What are the best practices for culturing the Human MELTF Stable Cell Line - A375 to ensure data reproducibility in experiments?

A: Ensuring reproducibility involves strict aseptic conditions, careful thawing, and handling. Avoiding excessive alkalinity and adhering to recommended culture conditions are crucial.

What ethical considerations should be kept in mind while using the Human MELTF Stable Cell Line - A375 in research?

A: It's essential to use A375 cells strictly for laboratory research purposes, respecting the ethical guidelines that prohibit their use in human or animal therapeutic applications, consumption, or diagnostics.

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