Cat.No. : CSC-RR0116 Host Cell: PC-3
| Cat. No. | CSC-RR0116 |
| Description | The PC-3 line was initiated from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62-year-old male Caucasian. The cells exhibit low acid phosphatase and testosterone-5-alpha reductase activities. The GFP Stable Cell Line-PC-3 constitutively expresses GFP. |
| Host Cell | PC-3 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Recommended Medium | Inquiry for instruction of culturing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Most tumors elevate their glucose metabolism to satisfy greater energy, biosynthesis, and redox requirements. The researchers have made significant strides in targeting cancer cell metabolic dependencies to develop new therapies. They discovered that hexokinase 2 (HK2), crucial for integrating glycolysis activation and apoptosis resilience, is regulated by K63-linked ubiquitination through HectH9. Using stable isotope tracers and functional metabolic analyses, the team found that HectH9 deficiency disrupts tumor glucose metabolism and growth by inhibiting HK2. This pathway also influences cancer stem cell (CSC) expansion and chemoresistance. Histological studies revealed that HectH9 expression is upregulated in prostate cancer and correlates with disease progression. Their findings highlight HectH9 as a novel regulator of HK2 and cancer metabolism, suggesting that targeting HectH9 could effectively disrupt glycolysis and induce apoptosis for long-term tumor remission.
Figure 1. HectH9 is a novel glucose metabolism regulator, with data on gene alterations, cell viability, ECAR, metabolite levels, and metabolic pathway changes in PC-3 cells. The GFP Stable Cell Line-PC-3 is used for tracking and analyzing cancer cell behavior and metabolic processes, including the effects of gene knockdowns on glycolysis and mitochondrial localization. (Lee HJ, et al., 2019)
A: The GFP Stable Cell Line-PC-3 is primarily used for studying cancer biology, particularly in the context of prostate cancer. The green fluorescent protein (GFP) expression allows researchers to visually track cell behavior, migration, and interaction in real-time, facilitating the study of tumor progression, metastasis, and the effects of anti-cancer drugs.
A: In the GFP Stable Cell Line-PC-3, GFP expression enables the visualization of cells under fluorescent microscopy without the need for additional staining procedures. This allows for the observation of live cell processes in real-time, including cell division, migration, and apoptosis, which are crucial for understanding the dynamics of cancer cells.
A: Yes, the GFP Stable Cell Line-PC-3 can be utilized in co-culture experiments to study interactions between cancer cells and other cell types, such as immune cells or endothelial cells. The GFP labeling allows for the easy differentiation of the PC-3 cells from other cell types under fluorescence microscopy, enabling researchers to analyze the effects of cell-cell interactions on cancer progression and immune response.
A: When maintaining the GFP Stable Cell Line-PC-3, researchers should ensure that the cells are cultured in appropriate media with the correct antibiotics to select for GFP-expressing cells. It's also important to monitor GFP expression regularly to ensure the stable expression of the fluorescent protein. Avoiding over-confluency is crucial to prevent changes in cell behavior and GFP expression levels.
A: The GFP Stable Cell Line-PC-3 is an invaluable tool in drug discovery, especially for identifying and characterizing compounds that can inhibit prostate cancer cell growth and metastasis. By tracking changes in GFP expression and cell morphology, researchers can assess the efficacy of drug candidates in real-time, enabling the rapid screening of potential therapeutic agents.
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GFP expression in PC-3 cells allows for easy tracing of cell lineages and monitoring of cell fate decisions in experimental setups.
The PC-3 GFP Stable Cell Line is excellent for phenotypic analysis, as the GFP tag can be attached to various proteins to study their functions in prostate cancer cells.
The GFP tag on PC-3 cells enhances the capability for multiparameter flow cytometry, enabling detailed cell cycle and apoptosis studies.
The GFP Stable Cell Line - PC-3 is suitable for studying drug uptake and intracellular trafficking patterns, which are important for understanding drug efficacy.
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