Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT2634
Target Gene : E2F1 Host Cell : PC3
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT2634 |
| Description | This cell is a stable cell line with a homozygous knockout of human E2F1 using CRISPR/Cas9. |
| Target Gene | E2F1 |
| Host Cell | PC3 |
| Host Cell Species | Homo sapiens (Human) |
| Size | 1 vial (10^6 cell/vial) |
| Validation | Sequencing |
| Storage | Liquid nirtogen |
| Shipping | Dry ice package |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | E2F1 E2F transcription factor 1 [ Homo sapiens ] |
| Gene Symbol | E2F1 |
| Synonyms | RBP3; E2F-1; RBAP1; RBBP3 |
| Gene Description | E2F transcription factor 1 |
| GeneID | 1869 |
| Uni ProtID | Q01094 |
| mRNA Refseq | NM_005225.2 |
| Protein Refseq | NP_005216.1 |
| Chromosome Location | 20q11.2 |
| Function | DNA binding; DNA binding; core promoter binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; transcription corepressor activity; transcription factor binding; |
| Pathway | Activation of BH3-only proteins, organism-specific biosystem; Activation of NOXA and translocation to mitochondria, organism-specific biosystem; Activation of PUMA and translocation to mitochondria, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Assembly of the pre-replicative complex, organism-specific biosystem; Association of licensing factors with the pre-replicative complex, organism-specific biosystem; |
| MIM | 189971 |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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It is very convenient to operate according to the instructions and improve the efficiency of the experiment.
The Human E2F1 Knockout Cell Line provides a clear procedure for the test, including usage, analysis of results, and precautions. This greatly simplifies laboratory procedures and provides sensitive and accurate results.
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