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Human E2F1 Knockout Cell Line-PC3

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT2634

Target Gene :   E2F1 Host Cell :   PC3

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT2634
Description This cell is a stable cell line with a homozygous knockout of human E2F1 using CRISPR/Cas9.
Target Gene E2F1
Host Cell PC3
Host Cell Species Homo sapiens (Human)
Size 1 vial (10^6 cell/vial)
Validation Sequencing
Storage Liquid nirtogen
Shipping Dry ice package
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name E2F1 E2F transcription factor 1 [ Homo sapiens ]
Gene Symbol E2F1
Synonyms RBP3; E2F-1; RBAP1; RBBP3
Gene Description E2F transcription factor 1
GeneID 1869
Uni ProtID Q01094
mRNA Refseq NM_005225.2
Protein Refseq NP_005216.1
Chromosome Location 20q11.2
Function DNA binding; DNA binding; core promoter binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; transcription corepressor activity; transcription factor binding;
Pathway Activation of BH3-only proteins, organism-specific biosystem; Activation of NOXA and translocation to mitochondria, organism-specific biosystem; Activation of PUMA and translocation to mitochondria, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Assembly of the pre-replicative complex, organism-specific biosystem; Association of licensing factors with the pre-replicative complex, organism-specific biosystem;
MIM 189971
Quick Inquiry

Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Very handy

It is very convenient to operate according to the instructions and improve the efficiency of the experiment.

France

Perfect use experience

The Human E2F1 Knockout Cell Line provides a clear procedure for the test, including usage, analysis of results, and precautions. This greatly simplifies laboratory procedures and provides sensitive and accurate results.

United States

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