Cat.No. : CSC-RO1013 Host Cell: PC3
Size: >1x10^6 cells/vial Validation: T7 Endonuclease I assay
| Cat. No. | CSC-RO1013 |
| Description | PC3-Cas9 cell line is engineered to stably overexpress Cas9 nuclease. The Cas9 nuclease in PC3-Cas9 cell line has been functionally validated using T7 Endonuclease I assay. In combination with separately transfected sgRNAs, PC3-Cas9 cell line can be used to efficiently generate targeted genomic modifications including gene knockout, gene knockin, gene mutagenesis, gene tagging etc. It is also an ideal cell line model for sgRNA screening and validation, either individually or in pools. |
| Introduction | Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology that contains two essential components: a guide RNA (gRNA) to match a target gene, and the Cas9 (CRISPR-associated protein 9) endonuclease which causes a double-stranded DNA break, allowing modifications to the genome via nonhomologous end joining (NHEJ) or homology-directed repair (HDR). |
| Target Gene | Cas9 |
| Host Cell | PC3 |
| Host Cell Species | Homo sapiens (Human) |
| Product Type | Cas9 overexpression stable cell line |
| Applications | 1) CRISPR genome editing, such as gene knockout (KO), gene knockin (KI), gene mutagenesis, gene tagging etc. 2) High-throughput sgRNA screening and validation |
| Quality Control | 1) T7E1 assay 2) Mycoplasma detection |
| Size Form | One vial of frozen cells, typically >1x10^6 cells/vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Metastasis is a key factor in the progression of prostate cancer (PCa), particularly when cancer cells spread to bone tissue. Understanding the molecular mechanisms that drive this process is critical for developing new treatments. The researchers focused on MAP4K4, identifying it as a crucial gene in PCa metastasis. They demonstrated that deleting MAP4K4 or inhibiting its kinase, HGK, impaired the migration and clonogenic abilities of metastatic PCa cells. Their findings revealed that HGK depletion alters cell spreading and focal adhesion, disrupting the cell's response to TNF-α stimulation and chemoattractants. Additionally, high levels of HGK correlated with poor prognosis in PCa patients, positioning it as a potential biomarker for aggressive cancer phenotypes. The researchers utilized the PC3 Cas9 stable cell line to perform targeted gene knockdowns.
Figure 1. The researchers investigated the impact of HGK down-regulation on prostate cancer cell migration and adhesion. Using the PC3 Cas9 stable cell line, they employed Western blotting to confirm HGK depletion and performed migration and wound healing assays. Their results showed that HGK silencing reduced migration and enhanced adhesion in PCa cells. (Garcia-Garcia S, et al., 2021)
A: The application of the Cas9 Stable Cell Line - PC3 in prostate cancer research includes modeling gene mutations in prostate cancer, studying the effects of Cas9-mediated gene editing on cell proliferation, apoptosis, and invasiveness, and developing novel treatment strategies for prostate cancer.
A: The Cas9 Stable Cell Line - PC3 assists in discovering new therapeutic targets in drug screening by creating cell models with specific gene mutations through Cas9-mediated gene editing, which helps in screening and validating potential drug targets, thus accelerating the drug development process.
A: The role of the Cas9 Stable Cell Line - PC3 in studying genomic stability in prostate cancer cells is to investigate the function of genes related to genomic stability through Cas9-mediated gene editing, which is crucial for understanding the molecular mechanisms of prostate cancer and developing new treatment strategies.
A: The role of the Cas9 Stable Cell Line - PC3 in studying signal transduction in prostate cancer cells is to investigate key genes in specific signaling pathways through Cas9-mediated gene editing, which helps reveal the signaling networks of prostate cancer cells and potential therapeutic intervention points.
A: The role of the Cas9 Stable Cell Line - PC3 in studying invasiveness and metastasis in prostate cancer cells is to investigate genes that affect cell migration and invasion through Cas9-mediated gene editing, which is significant for understanding the mechanisms of prostate cancer spread and developing new treatment strategies.
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The Cas9 Stable Cell Line - PC3, with Cas9 stably expressed, enables researchers to immediately commence CRISPR/Cas9-mediated gene editing, eliminating the need for additional time to construct or validate Cas9 expression systems, significantly speeding up the experimental process.
The Cas9 Stable Cell Line - PC3 provides a stable gene editing environment, reducing experimental variability caused by fluctuations in Cas9 expression levels, enhancing the reproducibility and reliability of experimental results.
As there is no need to construct and validate Cas9 expression systems for each experiment, the Cas9 Stable Cell Line - PC3 significantly saves researchers' time and resources, allowing them to allocate more effort and funding to other areas of research.
As a prostate cancer model, the Cas9 Stable Cell Line - PC3 is suitable for a wide variety of gene function studies, drug screening, and disease mechanism research, providing researchers with a powerful tool to deeply understand the genetics and biology of prostate cancer.
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