Cat.No. : CSC-RO0702 Host Cell: LL/2 (LLC1)
| Cat. No. | CSC-RO0702 |
| Description | This cell line is engineered to stably overexpress mouse Fap in LL/2 which is a murine Lewis lung carcinoma cell line and is commonly used in the field of oncology. |
| Gene | Fap |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | LL/2 (LLC1) |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: In drug toxicity studies, the Mouse Fap Stable Cell Line - LL/2 can be used to assess the potential hepatotoxicity of drugs. By monitoring the expression of FAP and cell viability after drug treatment, researchers can evaluate the safety of drugs on the liver, which is significant for drug development and toxicity testing.
A: The role of the Mouse Fap Stable Cell Line - LL/2 in studying liver fibrosis is reflected in its ability to model the activation process of hepatic stellate cells (HSCs). As FAP is a marker of HSC activation, studying the LL/2 cell line allows scientists to better understand the mechanisms of liver fibrosis development, providing foundational data for the development of anti-fibrotic therapies.
A: The value of the Mouse Fap Stable Cell Line - LL/2 in studying liver cancer lies in its ability to model the growth and invasive characteristics of liver cancer cells. By studying the role of FAP in liver cancer cells, scientists can explore the molecular mechanisms of liver cancer, which is significant for developing new anticancer strategies and treatments.
A: The Mouse Fap Stable Cell Line - LL/2 serves as a research tool to help scientists investigate the molecular mechanisms of liver cell regeneration. By observing the expression changes of FAP following liver cell injury, key signaling pathways involved in the regeneration process can be revealed, which is crucial for developing new strategies for treating liver diseases.
A: The Mouse Fap Stable Cell Line - LL/2 is derived from mouse hepatocytes. This cell line is engineered to stably express the hepatocyte growth factor (HGF) receptor, known as fibroblast activation protein (FAP).
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The "Mouse Fap Stable Cell Line - LL/2" ensures high specificity in the expression of the FAP gene, which is crucial for studying the role of FAP protein in the tumor microenvironment.
Through continuous selection markers, the "Mouse Fap Stable Cell Line - LL/2" maintains a stable phenotype over multiple cell culture generations, which is important for the reproducibility of experimental results.
The genetic background of the "Mouse Fap Stable Cell Line - LL/2" allows researchers to easily perform further genetic manipulations to explore complex biological questions.
The "Mouse Fap Stable Cell Line - LL/2" has been widely used in scientific research, with a wealth of literature available, providing a reliable reference for new studies.
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