Cat.No. : CSC-RR0525 Host Cell: LL/2 (LLC1)
| Cat. No. | CSC-RR0525 |
| Description | This cell line is engineered to stably overexpress GFP reporter gene. LLC is a murine lewis lung carcinoma cell line, which has been widely used in laboratory research. This cell line is a useful tool for fluorescent tracking of LLC cells. |
| Gene | GFP |
| Host Cell | LL/2 (LLC1) |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
While ICG fluorescence imaging has been employed for lung cancer detection, there remains a lack of agreement on the optimal method for administering indocyanine green (ICG) injections. Researchers investigated the optimal dose and timing of indocyanine green (ICG) injection for lung cancer detection using animal models and clinical evaluation. Twenty C57BL/6 mice with footpad cancer and thirty-three rabbits with VX2 lung cancer received ICG injections at varying doses and time intervals. In clinical studies, fifty-one lung cancer patients underwent surgery after ICG injection. Results showed that injecting 2 mg/kg ICG 12 hours before surgery yielded optimal detection rates. ICG successfully detected 37 of 39 cases with a consolidation-to-tumor ratio > 50%, suggesting its potential in lung cancer diagnosis. Further research is warranted to enhance fluorescent agents targeting lung cancer.
Figure 1. Time and dose optimization of ICG for tumor detection in mice model with footpad tumor was conducted by researchers. NIR fluorescence imaging and quantification of TNR in tumor tissues were performed using Image J software. The Lewis lung carcinoma–green fluorescent protein (LLC-GFP) cells are sourced from Creative Biogene. (Jeon OH, et al., 2023)
A: To ensure the stability and expression level of GFP in the LLC GFP Reporter Cell Line, stable transfection methods were employed to integrate the GFP reporter gene into LLC cells. The clonal selection was then performed to isolate cell lines with stable and consistent GFP expression levels. Regular monitoring of GFP fluorescence using fluorescence microscopy or flow cytometry confirmed sustained expression levels over time.
A: Functional characterization of GFP expression in the LLC GFP Reporter Cell Line involved assessing its responsiveness to regulatory elements and its involvement in cellular processes relevant to the study. This included studying the activation of specific regulatory elements, such as promoters or enhancers, and monitoring changes in GFP fluorescence in response to stimuli or treatments. Additionally, the role of GFP in cellular processes such as protein localization, organelle tracking, or cell cycle dynamics was investigated using live-cell imaging and fluorescence microscopy.
A: Stable transfection methods were employed to integrate the mCherry and luciferase reporter genes into A20 cells, ensuring stable and sustained expression. Clonal selection isolated cell lines with consistent expression levels. Regular monitoring using fluorescence microscopy for mCherry and luciferase assays confirmed stable expression over time.
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The GFP Reporter Cell Line consistently provides robust signals, ensuring reproducible results and accurate interpretation of experimental outcomes. From studying tumor microenvironment interactions to evaluating treatment responses, this cell line serves as an invaluable resource for unraveling the complexities of lung cancer biology and developing novel therapeutic strategies.
The GFP Reporter Cell Line in LLC cells offers clear and vibrant GFP expression, enabling detailed visualization and analysis of cellular processes in my lung cancer research.
With GFP fluorescence, this cell line facilitates comprehensive investigation of tumor growth, metastasis, and response to therapy, advancing our understanding of lung cancer biology. Its stable GFP expression simplifies experimental workflows, allowing for efficient data collection and analysis in lung cancer research.
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