ZsYellow1 Reporter Cell Line - LL/2 (LLC1)

ZsYellow1 is a great option for multicolored applications because of its true yellow emission. It is ideal for live-cell imaging since it can be found inside cells without the use of other substances or agents. Human codon optimization has been applied to the ZsYellow1 gene to improve translation in mammalian cells. This has led to improved solubility, brighter emission, and faster chromophore maturation (8–12 hours). Protein synthesis and turnover, protein trafficking, gene induction, and cell lineage can all be seen, tracked, and quantified in cells using the fluorescent protein ZsYellow1 as a molecular tag or reporter. The lungs of C57BL mice bearing tumors brought on by the implantation of primary Lewis lung carcinoma are used to create LLC. Methotrexate causes these cells to become sensitive, although they are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea. Although LLC cells have a low propensity for metastasis in mice, they have a significant tumorigenicity. The LLC cell line is widely used as a model for tumor metastasis and is crucial for understanding how chemotherapy medications work.