Cat.No. : CSC-RR0526 Host Cell: LL/2 (LLC1)
| Cat. No. | CSC-RR0526 |
| Description | This cell line is engineered to stably overexpress ZsYellow1 reporter gene. LLC is a murine lewis lung carcinoma cell line, which has been widely used in laboratory research. This cell line is a useful tool for fluorescent tracking of LLC cells. |
| Gene | ZsYellow1 |
| Host Cell | LL/2 (LLC1) |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The ZsYellow1 gene in ZsYellow1 Reporter Cell Line - LLC is integrated into the genome through stable transfection technology. The expression level has been verified through multiple passages and remains stable. This stability is particularly important for long-term experiments and high-throughput screening, ensuring reproducibility and data consistency across different time points and batches of experiments.
A: ZsYellow1 Reporter Cell Line - LLC's ZsYellow1 reporter gene encodes a yellow-green fluorescent protein suitable for live cell imaging experiments. ZsYellow1 has the characteristics of high brightness and slow photobleaching, and can monitor cell activities and protein localization in real-time in living cells. Its excellent fluorescence properties allow this cell line to perform well in dynamic imaging and long-term tracking experiments, providing clear images and reliable data.
A: ZsYellow1 Reporter Cell Line - LLC is ideal for high-throughput drug screening experiments due to its high sensitivity and stability of the reporter gene. After drug treatment in this cell line, the expression changes of ZsYellow1 fluorescent protein can be quickly read out by high-throughput fluorescence detection equipment, greatly improving the screening efficiency. Its applicability has been verified on a variety of drug screening platforms, and it can meet the needs of high-throughput screening and provide rapid and reliable drug effect evaluation.
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The logistics was fast, the packaging was intact, and the ZsYellow1 reporter cell line is in good condition. The cells' yellow fluorescence is very bright, making them particularly suitable for multicolor imaging applications. During the live cell imaging process, there is no need to add additional substances and the observation effect is clear. The translation efficiency of cells is high, the synthesis and maturation of fluorescent proteins is fast, and the experimental process becomes smoother. Overall, I am very satisfied with the product quality and logistics service.
Very satisfied with this ZsYellow1 reporter cell line. The cells performed stably during the culture process, and the brightness and solubility of the fluorescent protein were excellent. After optimization of the ZsYellow1 gene, it is more suitable for mammalian cells and the effect is significantly improved. Coupled with the high tumorigenicity of LLC cell lines, it is very helpful to study tumor metastasis and chemotherapeutic drug mechanisms.
The emission spectrum of ZsYellow1 fluorescent protein is very pure and suitable for multi-color fluorescence imaging. Human codon optimization enables higher translation efficiency, brighter fluorescence, and good staining effects in mammalian cells. The results are remarkable for observing protein synthesis, transport, and cell lineage. Although the LLC cell line has low metastasis in mice, it has strong tumor formation ability and is suitable as a model for tumor metastasis research. The whole shopping experience is very pleasant and worth recommending
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