GFP Reporter Cell Line - Huh7

The HuH-7 cell line was established in 1982 from a well-differentiated hepatocyte-derived carcinoma cell line originally derived from a liver tumor of a 57-year-old Japanese man. This cell line was established by Nakabayshi, H. and Sato, J.. Huh-7 is an immortal cell line composed of epithelioid tumorigenic cells. Most Huh-7 cells have chromosome numbers between 55 and 63 (pattern 60) and are highly heterogeneous. Huh7 cells have played an important role in hepatitis C research. Until 2005, it was impossible to culture hepatitis C in the laboratory. The introduction of the Huh7 cell line allows screening of drug candidates against laboratory-grown hepatitis C virus and allows the development of new drugs against hepatitis C. The GFP reporter cell line - Huh7 is a powerful tool for monitoring gene expression in real time. The cells, derived from the human liver cancer cell line Huh7, were genetically engineered to express green fluorescent protein (GFP) when a specific promoter is activated. After transfection with a specific gene of interest, the GFP reporter in these cells fluoresces green when exposed to light in the blue to ultraviolet range. This characteristic luminescence can serve as an indicator of gene expression, allowing researchers to visually monitor the transcriptional activity of genes of interest qualitatively and quantitatively.