GFP/Luc Reporter Cell Line - Huh7

Huh7 is an immortalized cell line that can be grown in the laboratory for research purposes. Huh7 is a well-differentiated hepatocyte-derived cancer cell line originally derived from a liver tumor in a 57-year-old Japanese man in 1982. These cells adhere to the surface of a flask or plate and typically grow as a 2D monolayer. Although containing many mutations and INDELS, it is noteworthy that Huh7 cells harbor point mutations in the p53 gene. Huh-7 is highly sensitive to hepatitis C virus (HCV) and is often used as a model to study liver cancer and HCV. This cell line can be used in HCV replicon systems, allowing the production of infectious HCV particles in vitro and the development of drugs against HCV. GFP/Luc Reporter Cell Line - Huh7 is engineered to stably express the genes encoding GFP and luciferase. The main advantage of this dual reporter system is that detection of GFP can be immediately visualized under a fluorescence microscope for cell sorting and tracking, while the luciferase allows non-invasive imaging of cells and animals in the bioluminescent mode, enabling long-term monitoring. Additionally, it provides the ability to generate quantitative data, making it a powerful tool for high-throughput screening applications.