Human CRBN Knockout Cell Line-Huh7

Name: Human CRBN Knockout Cell Line-Huh7
SKU: CSC-RT2819
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2819

Target Gene : CRBN Host Cell : Huh7

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2819
Description	This cell is a stable cell line with a homozygous knockout of human CRBN using CRISPR/Cas9.
Target Gene	CRBN
Host Cell	Huh7
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CRBN cereblon [ Homo sapiens ]
Gene Symbol	CRBN
Synonyms	CRBN; cereblon; mental retardation, non syndromic, autosomal recessive, 2A , MRT2A; protein cereblon; protein x 0001; MRT2; MRT2A; MGC27358; DKFZp781K0715;
Gene ID	51185
Uni Prot ID	Q96SW2
m RNA Refseq	BC017419
Chromosome Location	3p26.3
Function	ATP-dependent peptidase activity; protein binding;
MIM	609262

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