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Cat.No. : CSC-RR0506 Host Cell: Huh7
| Cat. No. | CSC-RR0506 |
| Description | This cell line is engineered to stably overexpress luciferase reporter gene. Huh7 cell line is a human liver cancer cell line that has been widely used in laboratory research. This cell line is a useful tool for Huh7 tracking. |
| Gene | Luciferase |
| Host Cell | Huh7 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
More than 70 million people globally are chronically infected with the hepatitis C virus (HCV), which is the main cause of liver cancer in the majority of those nations. The Luciferase Reporter Cell Line - Huh7 was employed by the researchers to examine the dynamics of Hepatitis C virus (HCV) infection. By utilizing gene expression profiling on several Huh7 variants, 34 potential host variables that impact HCV permissiveness were discovered. Seven candidates, including THAP7 and NR0B2, were validated by knock-down tests that demonstrated increased HCV replication upon overexpression. Notably, the ideal expression level of NR0B2 in highly permissive cells indicates a delicate balance impacting the metabolism of cholesterol and bile acid, which is essential for the regulation of HCV replication. Our knowledge of viral pathogenesis and possible treatment targets is advanced by these findings, which clarify important host genes influencing HCV tropism in Huh7 cells.
Figure 1. The researchers demonstrated the efficacy of Luciferase Reporter Cell Line - Huh7 in assessing HCV replication. siRNA knockdown of host factors reduced viral replication in both infection and replicon models. (Dächert C, et al., 2019)
A: The Huh7 Luciferase reporter cell line is known for its high level and stable luciferase activity and is suitable for a variety of research applications. This cell line has been carefully selected and optimized to ensure stable expression of luciferase within cells. Whether it is a short-term experiment or a long-term culture, the luciferase activity remains consistent and will not decrease significantly as the number of passages increases. This stability is crucial to ensure the reliability and reproducibility of experimental data. In addition, each batch of cell lines undergoes strict quality control and verification to ensure high quality and consistency.
A: When conducting drug screening experiments using Huh7 Luciferase reporter cell lines, it is crucial to optimize experimental conditions. First, ensure that the cells are growing in optimal conditions and select appropriate culture media and culture conditions, including temperature, CO2 concentration, etc. Secondly, the addition time and concentration of the drug must be precisely controlled. It is recommended to use the gradient dilution method to determine the optimal working concentration. At the same time, use high-quality luminescent substrates (such as D-luciferin) and perform detection at appropriate time points to ensure the peak of the luminescent signal. For the measurement of luminescence signals, use a luminescence detector with high sensitivity and low background noise, and set reasonable measurement parameters (such as integration time). Additionally, ensure that each experiment has appropriate positive and negative controls to verify the actual effect of the drug and the accuracy of the experiment.
A: When analyzing luminescence data from a Huh7 Luciferase reporter cell line, ensuring the accuracy and reproducibility of your results requires several critical steps. First, perform data preprocessing, including removing outliers and background noise. Secondly, perform normalization processing and use internal standard or standard curve method to eliminate systematic errors between experiments. Then, the experimental data were statistically analyzed and the mean and standard deviation were calculated to evaluate the variability of the data. In order to improve the reliability of the data, it is recommended to repeat the experiment multiple times and use charts or images to visually display the data for easy observation and comparison. Additionally, experimental conditions and parameters were recorded in detail to allow for experimental replication and result verification when needed.
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The merchant's product logistics is very fast, and it only takes three days from placing the order to receiving the goods. The packaging was also very careful and there was no damage. After conducting experiments using Huh7 cells, I found that the growth status of these cells was very consistent and showed high stability in gene expression. This kind of cell line also has a strong differentiation ability and is suitable for studying the relationship between liver cancer and HCV infection.
United States
03/02/2020
This Luciferase reporter cell line Huh7 cell line is really good, I use them to study gene expression regulation. The cells have good attachment and growth properties and their shape remains very stable. The customer service of the merchant is also very good, and some minor problems I had during use were solved in a timely manner.
French
02/02/2024
The logistics are very fast and the packaging is very strong. Huh7 cells performed well in my experiments, with stable cell growth and gene expression. Very satisfied. Moreover, Huh7 cells are also widely used in xenograft animal models, which provides more possibilities for my research. The merchant's product quality and service are worthy of praise.
French
06/27/2020
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