Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0113
Target Gene : Kat2b Host Cell : HBZY-1
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0113 |
| Description | HBZY-1-Kat2b cell line is a stable cell line with a homozygous knockout of Kat2b |
| Target Gene | Kat2b |
| Host Cell | HBZY-1 |
| Host Cell Species | Rattus norvegicus (Rat) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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I cannot emphasize enough how valuable Traf6 Knockout Cell Line-HBZY-1 has been for unraveling the functional significance of specific genes. Its exceptional performance has enabled me to uncover critical insights into biological processes.
Kat2b Knockout Cell Line-HBZY-1 is a true gem in the world of genetic research. Its remarkable knockout efficiency and reliable performance have allowed me to unlock the secrets of gene function with unparalleled precision.
I thoroughly have deep impression with the performance of Kat2b Knockout Cell Line-HBZY-1. Its consistent knockout outcomes have provided me with a solid foundation for studying gene function and unraveling complex biological pathways.
Using Kat2b Knockout Cell Line-HBZY-1 has been a breeze. Its user-friendly interface and exceptional knockout efficiency have simplified the gene editing process, making it accessible to researchers of all skill levels.
I cannot stress enough the impact of Kat2b Knockout Cell Line-HBZY-1 on my research. Its high success rate and reproducible knockout results have propelled my investigations and led to significant discoveries in the field.
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