Cat.No. : CSC-RT0106
Host Cell: HBZY-1 Target Gene: Klf6
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0106 |
| Description | HBZY-1-Klf6 cell line is a stable cell line with a homozygous knockout of Klf6 |
| Target Gene | Klf6 |
| Host Cell | HBZY-1 |
| Host Cell Species | Rattus norvegicus (Rat) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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I am truly amazed by the effectiveness of Klf6 Knockout Cell Line-HBZY-1. Its outstanding knockout efficiency and reliable performance have been instrumental in unraveling the intricate mechanisms underlying gene function.
Using Klf6 Knockout Cell Line-HBZY-1 has been a outstanding competent helper for my research. Its high success rate and consistent knockout outcomes have provided me with a solid foundation for studying gene function and its implications in various biological processes.
Klf6 Knockout Cell Line-HBZY-1 has simplified the gene editing process for me. Its user-friendly interface, combined with its exceptional knockout efficiency, has made it a go-to tool for generating precise genetic modifications.
I cannot emphasize enough the value of Klf6 Knockout Cell Line-HBZY-1 in my studies. Its exceptional performance and reproducible knockout results have allowed me to make significant strides in understanding the functional impact of specific genes.
I wholeheartedly recommend Klf6 Knockout Cell Line-HBZY-1 to fellow researchers. Its outstanding performance, ease of use, and consistent knockout outcomes have accelerated my research progress and enriched my scientific investigations.
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