Klf6 Knockout Cell Line-HBZY-1

Name: Klf6 Knockout Cell Line-HBZY-1
SKU: CSC-RT0106
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0106

Target Gene : Klf6 Host Cell : HBZY-1

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0106
Description	HBZY-1-Klf6 cell line is a stable cell line with a homozygous knockout of Klf6
Target Gene	Klf6
Host Cell	HBZY-1
Host Cell Species	Rattus norvegicus (Rat)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Klf4 Kruppel-like factor 4 (gut) [ Rattus norvegicus ]
Gene Symbol	Klf4
Synonyms	GKLF
Gene Description	Kruppel-like factor 4 (gut)
GeneID	114505
Uni ProtID	Q923V7
mRNA Refseq	NM_053713.1
Protein Refseq	NP_446165.1
Chromosome Location	5q24
Function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; core promoter proximal region sequence-specific DNA binding; core promoter proximal region sequence-specific DNA binding; double-stranded DNA binding; phosphatidylinositol 3-kinase regulator activity; sequence-specific DNA binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor recruiting transcription factor activity; sequence-specific DNA binding transcription factor recruiting transcription factor activity; transcription factor binding; transcription regulatory region DNA binding; transcription regulatory region DNA binding; zinc ion binding;
Pathway	Developmental Biology, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Synthesis, Secretion, and Deacylation of Ghrelin, organism-specific biosystem; Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystem;

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Amazed by the effectiveness

I am truly amazed by the effectiveness of Klf6 Knockout Cell Line-HBZY-1. Its outstanding knockout efficiency and reliable performance have been instrumental in unraveling the intricate mechanisms underlying gene function.

United Kingdom

2023-02-23

High success rate

Using Klf6 Knockout Cell Line-HBZY-1 has been a outstanding competent helper for my research. Its high success rate and consistent knockout outcomes have provided me with a solid foundation for studying gene function and its implications in various biological processes.

United States

2023-04-11

Exceptional knockout efficiency

Klf6 Knockout Cell Line-HBZY-1 has simplified the gene editing process for me. Its user-friendly interface, combined with its exceptional knockout efficiency, has made it a go-to tool for generating precise genetic modifications.

United States

2020-08-23

Reproducible knockout results

I cannot emphasize enough the value of Klf6 Knockout Cell Line-HBZY-1 in my studies. Its exceptional performance and reproducible knockout results have allowed me to make significant strides in understanding the functional impact of specific genes.

United States

2021-05-12

Enriched my scientific investigation

I wholeheartedly recommend Klf6 Knockout Cell Line-HBZY-1 to fellow researchers. Its outstanding performance, ease of use, and consistent knockout outcomes have accelerated my research progress and enriched my scientific investigations.

United States

2021-08-15

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Klf6 Knockout Cell Line-HBZY-1

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Amazed by the effectiveness

High success rate

Exceptional knockout efficiency

Reproducible knockout results

Enriched my scientific investigation

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