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Cat.No. : CSC-RT0112
Host Cell: HBZY-1 Target Gene: Traf6
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0112 |
| Description | HBZY-1-Traf6 cell line is a stable cell line with a homozygous knockout of Traf6 |
| Target Gene | Traf6 |
| Host Cell | HBZY-1 |
| Host Cell Species | Rattus norvegicus (Rat) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I cannot overstate the importance of Traf6 Knockout Cell Line-HBZY-1 in my research endeavors. Its outstanding performance and reproducible knockout results have paved the way for breakthrough discoveries and advanced our understanding of gene function.
France
01/27/2022
I wholeheartedly endorse Traf6 Knockout Cell Line-HBZY-1 to researchers seeking reliable and effective tools for gene editing. Its exceptional performance, ease of use, and consistent knockout outcomes have accelerated my research progress and enhanced the quality of my scientific investigations.
United States
07/19/2022
Traf6 Knockout Cell Line-HBZY-1 has truly exceeded my expectations. Its exceptional knockout efficiency and reliable performance have propelled my research to new heights, enabling groundbreaking discoveries.
United States
11/03/2022
I am highly impressed with the reliability of Traf6 Knockout Cell Line-HBZY-1. Its consistent knockout outcomes have provided me with confidence in the accuracy and reproducibility of my experimental results.
United States
02/23/2023
Using Traf6 Knockout Cell Line-HBZY-1 has been a outstanding tool for my research endeavors. Its ease of use and exceptional knockout efficiency have allowed me to achieve targeted gene modifications with ease.
United States
04/11/2023
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