Klf4 Knockout Cell Line-HBZY-1

Name: Klf4 Knockout Cell Line-HBZY-1
SKU: CSC-RT0107
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0107

Target Gene : Klf4 Host Cell : HBZY-1

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0107
Description	HBZY-1-Klf4 cell line is a stable cell line with a homozygous knockout of Klf4
Target Gene	Klf4
Host Cell	HBZY-1
Host Cell Species	Rattus norvegicus (Rat)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Klf4 Kruppel-like factor 4 (gut) [ Rattus norvegicus ]
Gene Symbol	Klf4
Synonyms	GKLF
Gene Description	Kruppel-like factor 4 (gut)
GeneID	114505
Uni ProtID	Q923V7
mRNA Refseq	NM_053713.1
Protein Refseq	NP_446165.1
Chromosome Location	5q24
Function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; core promoter proximal region sequence-specific DNA binding; core promoter proximal region sequence-specific DNA binding; double-stranded DNA binding; phosphatidylinositol 3-kinase regulator activity; sequence-specific DNA binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor recruiting transcription factor activity; sequence-specific DNA binding transcription factor recruiting transcription factor activity; transcription factor binding; transcription regulatory region DNA binding; transcription regulatory region DNA binding; zinc ion binding;
Pathway	Developmental Biology, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Synthesis, Secretion, and Deacylation of Ghrelin, organism-specific biosystem; Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystem;

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Greater accuracy

I cannot stress enough how valuable Klf6 Knockout Cell Line-HBZY-1 has been in my research endeavors. Its consistent and efficient knockout capabilities have enabled me to unravel the complexities of gene function with greater accuracy.

United States

2021-10-09

Elevated the quality

I fullheartedly recommend Klf6 Knockout Cell Line-HBZY-1 to fellow researchers. Its exceptional performance, ease of use, and reliable knockout results have elevated the quality and impact of my scientific investigations.

United States

2022-01-27

Reliable performance

Klf4 Knockout Cell Line-HBZY-1 has truly surpassed my expectations. Its exceptional knockout efficiency and reliable performance have empowered me to unravel the mysteries of gene function with precision and confidence.

Germany

2022-07-19

Solid foundation

I am impressed with the performance of Klf4 Knockout Cell Line-HBZY-1. Its reliable and reproducible knockout outcomes have provided me with a solid foundation for studying gene function and exploring complex biological pathways.

United States

2022-11-03

Simplified the gene editing process

Using Klf4 Knockout Cell Line-HBZY-1 has been a seamless experience. Its user-friendly design and exceptional knockout efficiency have simplified the gene editing process, allowing me to focus more on the scientific questions at hand.

United States

2023-02-23

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Klf4 Knockout Cell Line-HBZY-1

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Greater accuracy

Elevated the quality

Reliable performance

Solid foundation

Simplified the gene editing process

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