Cat.No. : CSC-RO0634 Host Cell: Bxpc-3
| Cat. No. | CSC-RO0634 |
| Description | This cell line is derived from Bxpc-3 and is engineered to stably overexpress Human CLDN18 isoform 2. |
| Gene | CLDN18-A2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Bxpc-3 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Claudin-18.2 (CLDN18.2) is a tight junction protein predominantly expressed in the gastric mucosa of healthy tissue. However, its expression becomes dysregulated in several malignancies, including pancreatic cancer, where it is often overexpressed on the surface of cancer cells. CLDN18.2 is now a promising therapeutic target, as demonstrated by the monoclonal antibody zolbetuximab, which has shown positive outcomes in clinical trials, improving overall survival and progression-free survival in combination with chemotherapy. Zolbetuximab induces immune effector functions like antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) by specifically binding to CLDN18.2, leading to the destruction of cancer cells.
Figure 1. The researchers inoculated BxPC-3~CLDN18.2 and MIA PaCa-2~CLDN18.2 cells into nude mice to create xenografts and monitored tumor growth kinetics by measuring tumor size twice weekly. (Türeci Ӧ, et al., 2018)
Our Human CLDN18-A2 Stable Cell Line - Bxpc-3 offers a reliable tool for researchers studying CLDN18.2's role in cancer progression and therapeutic intervention. This cell line, derived from human pancreatic cancer, is ideal for evaluating antibody efficacy, immune responses, and potential drug interactions.
A: By stably expressing CLDN18.2, this cell line can mimic the interactions between gastric cancer cells and the tumor microenvironment, providing an experimental platform for studying tumor cell proliferation, migration, and invasion.
A: By observing the expression of CLDN18.2 in Bxpc-3 cells, researchers can evaluate the effectiveness of immunotherapy strategies targeting CLDN18.2, such as CAR-T cell therapy or monoclonal antibody treatment.
A: This cell line can be used to screen potential drugs targeting CLDN18.2 by observing the effects of drugs on the expression and function of CLDN18.2, aiding in the discovery of new gastric cancer treatment drugs.
A: By regularly conducting genetic stability tests, such as Southern blot or real-time PCR, the copy number and expression levels of the CLDN18.2 gene can be monitored to ensure the stability of the cell line in long-term culture.
A: This cell line can be used to study the role of CLDN18.2 in the migration and invasion of gastric cancer cells, which is crucial for understanding the mechanisms of gastric cancer metastasis and developing anti-metastatic strategies.
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The Human CLDN18-A2 Stable Cell Line - Bxpc-3 specifically expresses CLDN18.2, providing an ideal model for studying the role of this protein in cancer.
This cell line is an important tool for studying the expression patterns of CLDN18.2 in cancers such as pancreatic and gastric cancer.
CLDN18.2 is associated with cell migration, and this cell line helps explore the function of CLDN18.2 in tumor metastasis.
This cell line aids in uncovering the role of CLDN18.2 in the pathological processes of cancer.
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