GFP/Luc Reporter Cell Line - BxPC-3

Highly aggressive pancreatic ductal adenocarcinoma (PDAC) mostly includes fibroblasts that interact closely with tumor cells is defined by notable desmoplasia. Researchers have demonstrated that these myofibroblasts play a crucial role in tumor progression through direct cell-cell contact, which enhances the epithelial-mesenchymal transition (EMT) of tumor cells. This transition facilitates invasion and correlates with poorer clinical outcomes. In studies utilizing co-culture systems, such as 3D Matrigel assays, the interaction between PDAC cells and fibroblasts has been shown to promote tumor spheroid formation and collective invasion, revealing the importance of the tumor microenvironment in cancer biology.

Figure 1. The researchers employed a human cytokine array to analyze the effects of direct versus indirect co-culture of BxPC-3 and pancreatic stellate cells, assessing cytokine levels and gene expression changes. (Chen YI, et al., 2022)

Investigating these dynamic relationships is made possible by Creative Biogene's GFP/Luc Reporter Cell Line, BxPC-3. Designed for advanced uses in tumour microenvironment research, this cell line lets researchers use bioluminescence to instantly see tumour progression and metastases. Using this cell line allows researchers to investigate the processes behind fibroblast-tumor cell interactions and evaluate the effects of different treatment approaches.