Cat.No. : CSC-RR0136 Host Cell: BxPC-3
| Cat. No. | CSC-RR0136 |
| Description | BxPC-3 was derived from a 61 year-old female with a primary adenocarcinoma of the pancreas. BxPC-3 cells produce mucin and the tumour produced in a nude mouse is moderately well to poorly differentiated like the primary adenocarcinoma. The GFP Stable Cell Line-BxPC-3 constitutively expresses GFP. |
| Background | The GFP Stable Cell Line in BxPC-3 cells is another model that utilizes GFP to study cellular processes in pancreatic cancer cells. BxPC-3 is a human pancreatic cancer cell line that is used for studying the biology of pancreatic cancer and for drug development. The stable expression of GFP in BxPC-3 cells allows for the visualization of pancreatic cancer cell behavior, such as cell growth, migration, and the response to potential therapeutic agents. This cell line is particularly useful for investigating the molecular mechanisms of pancreatic cancer development and for the development of targeted therapies against this aggressive cancer type. |
| Host Cell | BxPC-3 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Recommended Medium | Inquiry for instruction of culturing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Stromal cells impact tumor cells' various morphologies and biological activities via secreted substances or direct cell interaction. Pancreatic ductal adenocarcinoma (PDAC) is distinguished by significant mesenchymal growth, with fibroblasts being the main cell type. Researchers discovered that contact between myofibroblasts abundant around the tumor and tumor cells undergoing epithelial-mesenchymal transition (EMT) enhances tumor invasion and is associated with a poorer clinical outcome in PDAC patients. Direct cellular interaction between fibroblasts and tumor cells was observed in primary pancreatic tumors and circulating tumor microemboli (CTM), resulting in heterocellular aggregation. Overexpressed ATP1A1 binds and recombines with fibroblast ATP1A1 in tumor cells, causing calcium oscillations, NF-κB activation, and Activin A release. Inhibiting ATP1A1 expression or neutralizing Activin A secretion reduced tumor invasion and colonization. These findings shed light on the direct contact between tumor cells and fibroblasts in PDAC growth, suggesting a possible treatment opportunity to restrict metastasis by interfering with these intercellular linkages.
Figure 1. Direct interaction between tumor cells and stromal cells plays an important role in PDAC progression. To explore this process in depth, they used the RFP-labeled BxPC-3 pancreatic cancer cell line in 3D-Matrigel co-culture experiments with GFP-labeled human pancreatic stellate cells. (Chen YI, et al., 2022)
With Creative Biogene's GFP stably expressing BxPC-3 cell line, you can deeply explore the critical interactions between tumor cells and stromal cells in pancreatic ductal adenocarcinoma (PDAC). This cell line uses GFP labeling technology, enabling you to clearly observe the behavior and interaction of tumor cells in in vitro models, especially in 3D-Matrigel co-culture experiments, which significantly improves the accuracy and reproducibility of your results.
A: The GFP Stable Cell Line-BxPC-3 has been genetically engineered to stably express the green fluorescent protein (GFP) under the control of a promoter that ensures consistent expression levels. This modification does not alter the pancreatic cancer phenotype of the BxPC-3 cell line, allowing researchers to study the characteristics of pancreatic cancer cells while benefiting from the visual advantages of GFP expression. This enables real-time tracking of cellular processes and the assessment of tumorigenic potential without disturbing the inherent properties of the BxPC-3 cells.
A: By taking advantage of the GFP fluorescence, researchers can monitor the effects of chemotherapeutic agents on the GFP Stable Cell Line-BxPC-3 in vitro. The changes in GFP fluorescence intensity and cellular morphology under drug exposure can provide valuable insights into the cytotoxic effects and potential mechanisms of action of these agents. This cell line can be used to perform dose-response studies and to identify compounds that effectively inhibit pancreatic cancer cell growth or induce apoptosis.
A: To preserve the GFP Stable Cell Line-BxPC-3, it is crucial to use a slow freezing process with appropriate cryopreservation media containing dimethyl sulfoxide (DMSO) as a cryoprotectant. The cells should be frozen at a rate of -1°C per minute to avoid the formation of ice crystals that can damage the cells. Upon thawing, cells should be quickly transferred to a 37°C water bath and then diluted in pre-warmed culture medium to minimize the osmotic shock. These conditions help maintain the stability of GFP expression and ensure high cell viability post-thaw.
A: The GFP Stable Cell Line-BxPC-3 can be utilized in various assays designed to study cell migration and invasion, such as wound healing assays, transwell assays, and spheroid invasion assays. The GFP tag allows for the direct visualization of the cells, enabling the tracking of individual cell movements and the assessment of collective invasion properties. This can provide insights into the molecular mechanisms that drive the metastatic behavior of pancreatic cancer cells and help identify potential therapeutic targets.
A: The GFP Stable Cell Line-BxPC-3 can be employed to establish orthotopic or xenograft models of pancreatic cancer in immunodeficient mice. The GFP expression facilitates the in vivo tracking of tumor growth, metastasis, and response to therapy, as it allows for non-invasive imaging using fluorescence microscopy. This can greatly enhance the translational potential of preclinical studies by providing real-time, longitudinal data on tumor progression and therapeutic efficacy.
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The BxPC-3 GFP Stable Cell Line allows for the direct visualization of cells without the need for fixation or staining, which is beneficial for live cell imaging studies.
GFP expression in BxPC-3 cells facilitates rapid and efficient cell sorting using fluorescence-activated cell sorting (FACS), which is crucial for isolating specific cell populations.
We can use the BxPC-3 GFP Stable Cell Line to track the localization of proteins of interest in real-time, providing insights into cellular processes.
The stable integration of the GFP reporter in BxPC-3 cells ensures a consistent expression level, which is important for longitudinal studies over multiple passages.
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