GFP Stable Cell Line-BxPC-3

Stromal cells impact tumor cells' various morphologies and biological activities via secreted substances or direct cell interaction. Pancreatic ductal adenocarcinoma (PDAC) is distinguished by significant mesenchymal growth, with fibroblasts being the main cell type. Researchers discovered that contact between myofibroblasts abundant around the tumor and tumor cells undergoing epithelial-mesenchymal transition (EMT) enhances tumor invasion and is associated with a poorer clinical outcome in PDAC patients. Direct cellular interaction between fibroblasts and tumor cells was observed in primary pancreatic tumors and circulating tumor microemboli (CTM), resulting in heterocellular aggregation. Overexpressed ATP1A1 binds and recombines with fibroblast ATP1A1 in tumor cells, causing calcium oscillations, NF-κB activation, and Activin A release. Inhibiting ATP1A1 expression or neutralizing Activin A secretion reduced tumor invasion and colonization. These findings shed light on the direct contact between tumor cells and fibroblasts in PDAC growth, suggesting a possible treatment opportunity to restrict metastasis by interfering with these intercellular linkages.

Figure 1. Direct interaction between tumor cells and stromal cells plays an important role in PDAC progression. To explore this process in depth, they used the RFP-labeled BxPC-3 pancreatic cancer cell line in 3D-Matrigel co-culture experiments with GFP-labeled human pancreatic stellate cells. (Chen YI, et al., 2022)

With Creative Biogene's GFP stably expressing BxPC-3 cell line, you can deeply explore the critical interactions between tumor cells and stromal cells in pancreatic ductal adenocarcinoma (PDAC). This cell line uses GFP labeling technology, enabling you to clearly observe the behavior and interaction of tumor cells in in vitro models, especially in 3D-Matrigel co-culture experiments, which significantly improves the accuracy and reproducibility of your results.