GFP Stable Cell Line-SK-OV-3

Name: GFP Stable Cell Line-SK-OV-3
SKU: CSC-RR0112
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0112

Host Cell : SKOV-3 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RR0112
Description	The SK-OV-3 cell line was derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumour. SK-OV-3 cells are resistant to tumor necrosis factor and to several cytotoxic drugs including diphtheria toxin, cis- platinum and adriamycin. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells. The GFP Stable Cell Line-SK-OV-3 constitutively expresses GFP.
Host Cell	SKOV-3
Host Cell Species	Homo sapiens (Human)
Reporter Type	Fluorescent protein
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Recommended Medium	Inquiry for instruction of culturing
Storage	Liquid nitrogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Background

Case Study

Applications

Q & A

Customer Reviews

The stable cell line for GFP Green fluorescence protein (GFP), a popular marker in molecular and cellular biology research, is expressed in the cellular model SK-OV-3. The naturally occurring protein known as green fluorescent protein (GFP) is obtained from the jellyfish Aequorea victoria and fluoresces green when exposed to blue or ultraviolet light. Early in the 1990s, the GFP gene was first cloned and expressed in mammalian cells, completely changing the fields of live-cell tracking and cellular imaging.

A human ovarian cancer cell line called SK-OV-3 is frequently used in cancer research. Originally, these cells were obtained from ovarian cancer patients' ascites fluid.

Of all gynecologic tumors, ovarian cancer is the most prevalent and deadly. Three generations of anti-αFR CAR (αFR-ζ, αFR-28ζ, and αFR-28BBζ) were created by the researchers and transfected into NK-92 cells using lentivirus technology. When cocultured with αFR-positive ovarian cancer cells, NK-92 cells expressing αFR-28BBζ displayed increased antigen-specific cytotoxicity, proliferation, and decreased apoptosis. Their powerful capacity to kill cancer cells in vitro was proven by real-time cell analysis and live imaging. Using a mouse xenograft model, these cells effectively eliminated αFR-positive ovarian cancer cells, extending survival. This study demonstrates the therapeutic potential of NK-92 cells equipped with third-generation anti-αFR CAR in the treatment of ovarian cancer.

Figure 1 shows live cell imaging experiments demonstrating the killing of SK-OV-3-RFP cells by NK-92-αFR-28BBζ-GFP cells. (doi: 10.1097/CJI.0000000000000286) Figure 1. The researchers utilized Stable Cell Line-SK-OV-3 to visualize NK-92-αFR-28BBζ-GFP cell killing of SK-OV-3-RFP cells through live cell imaging. (Ao X, et al., 2019)

The GFP Stable Cell Line-SK-OV-3 offered by Creative Biogene facilitates experiments similar to the described study. These cells express stable GFP labeling, ideal for live cell imaging and cell interaction studies. Utilizing our cell line enables straightforward coculture experiments with SK-OV-3 cells and other cell types, allowing precise observation and analysis of cellular behaviors.

1. Cell imaging and tracking: Using GFP stable cell lines expressed in SK-OV-3 cells, cell imaging and tracking experiments were performed to explore biological processes such as cell morphology, migration, and proliferation, and provide visual data support for cancer research.

2. Gene expression analysis: By detecting the intensity and distribution of GFP fluorescence signals, gene expression levels, and cell functional states were evaluated to reveal the regulatory mechanisms and action pathways of different genes in SK-OV-3 cells.

3. Cell signal transduction: Using GFP labeling, the signal transduction pathways in SK-OV-3 cells were studied to explore the regulatory mechanisms of important biological processes such as cancer cell proliferation, metastasis, and drug resistance.

4. Drug screening and evaluation: Using GFP stable cell lines, drug screening, and evaluation experiments were performed to evaluate the effects of candidate drugs on the growth and survival of SK-OV-3 cells, providing a reference and basis for the development of anticancer drugs.

5. Cancer research: As a representative of human ovarian cancer cell lines, SK-OV-3 cells are of great significance in cancer research. By using GFP-stable cell lines, we can deeply explore the pathogenesis and treatment strategies of ovarian cancer and provide new ideas and methods for clinical treatment.

Customer Q&As

What is the GFP expression level of GFP Stable Cell Line-SK-OV-3 stable cell line? Does GFP expression remain stable during long-term culture?

A: Our GFP Stable Cell Line-SK-OV-3 has undergone strict screening and verification to ensure the stable expression of the GFP gene. Through methods such as fluorescence microscopy and flow cytometry, we verified the expression level of GFP and performed repeated tests at different time points and in different batches. The results showed that the cells could maintain stable GFP expression under appropriate culture conditions.

How does the SK-OV-3 background of this cell line affect the performance of the product? Are there any special considerations related to this cell line?

A: The impact of the SK-OV-3 cell line as a vector on the GFP stable cell line is considered to be minimal. However, we recommend that customers pay attention to the special growth requirements of the SK-OV-3 cell line during use, such as culture medium components and culture conditions, to ensure optimal expression and stability of the cells. We provide detailed training guides to help clients overcome potential issues.

Have the cell lines for this product been tested for contaminants? How to ensure the purity of cell lines?

A: Yes, our GFP Stable Cell Line-SK-OV-3 has undergone rigorous contaminant testing, including testing for common contaminants such as mycoplasma and bacteria. We use methods such as PCR and cell culture testing to ensure the purity of our cell lines. Our strict quality control procedures ensure that each batch of cell lines meets high standards of purity.

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Customer Reviews

Stability

During use, I found that the growth status of these cells was very stable, they always seemed to maintain a consistent growth rate, and their gene expression was also very stable. This is very important for my experiments as I need reliable cell models to conduct my research. The SK-OV-3 cell line not only provides stable cell growth, but also stable gene expression, which makes my experimental results more credible.

Canada

2024-04-08

Uniform growth

I used this SK-OV-3 GFP stable cell line to conduct a series of experiments and I was very satisfied with the results. The cells showed excellent stability in culture, with very consistent growth and stable gene expression. I observed that these cells exhibited bright green fluorescence under a fluorescence microscope, further demonstrating their stability and reliability.

French

2020-09-06

Gene expression

I did some experiments with this SK-OV-3 GFP stable cell line and the results were very satisfactory. These cells showed very good stability during the culture process, their growth status has been very healthy and stable, and their gene expression has also been maintained at a stable level. I observed that these cells showed bright green fluorescence under a fluorescence microscope, which indicated that the GFP gene was well expressed in these cells.

Germany

2023-05-28

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GFP Stable Cell Line-SK-OV-3

Cell Line Information

Cell Culture Information

Safety and Packaging

Background

Case Study

Applications

Q & A

Customer Reviews

What is the GFP expression level of GFP Stable Cell Line-SK-OV-3 stable cell line? Does GFP expression remain stable during long-term culture?

How does the SK-OV-3 background of this cell line affect the performance of the product? Are there any special considerations related to this cell line?

Have the cell lines for this product been tested for contaminants? How to ensure the purity of cell lines?

Stability

Uniform growth

Gene expression

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