Cat.No. : CSC-RR0112 Host Cell: SK-OV-3
| Cat. No. | CSC-RR0112 |
| Description | The SK-OV-3 cell line was derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumour. SK-OV-3 cells are resistant to tumor necrosis factor and to several cytotoxic drugs including diphtheria toxin, cis- platinum and adriamycin. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells. The GFP Stable Cell Line-SK-OV-3 constitutively expresses GFP. |
| Host Cell | SK-OV-3 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Recommended Medium | Inquiry for instruction of culturing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Of all gynecologic tumors, ovarian cancer is the most prevalent and deadly. Three generations of anti-αFR CAR (αFR-ζ, αFR-28ζ, and αFR-28BBζ) were created by the researchers and transfected into NK-92 cells using lentivirus technology. When cocultured with αFR-positive ovarian cancer cells, NK-92 cells expressing αFR-28BBζ displayed increased antigen-specific cytotoxicity, proliferation, and decreased apoptosis. Their powerful capacity to kill cancer cells in vitro was proven by real-time cell analysis and live imaging. Using a mouse xenograft model, these cells effectively eliminated αFR-positive ovarian cancer cells, extending survival. This study demonstrates the therapeutic potential of NK-92 cells equipped with third-generation anti-αFR CAR in the treatment of ovarian cancer.
Figure 1. The researchers utilized Stable Cell Line-SK-OV-3 to visualize NK-92-αFR-28BBζ-GFP cell killing of SK-OV-3-RFP cells through live cell imaging. (Ao X, et al., 2019)
The GFP Stable Cell Line-SK-OV-3 offered by Creative Biogene facilitates experiments similar to the described study. These cells express stable GFP labeling, ideal for live cell imaging and cell interaction studies. Utilizing our cell line enables straightforward coculture experiments with SK-OV-3 cells and other cell types, allowing precise observation and analysis of cellular behaviors.
A: Our GFP Stable Cell Line-SK-OV-3 has undergone strict screening and verification to ensure the stable expression of the GFP gene. Through methods such as fluorescence microscopy and flow cytometry, we verified the expression level of GFP and performed repeated tests at different time points and in different batches. The results showed that the cells could maintain stable GFP expression under appropriate culture conditions.
A: The impact of the SK-OV-3 cell line as a vector on the GFP stable cell line is considered to be minimal. However, we recommend that customers pay attention to the special growth requirements of the SK-OV-3 cell line during use, such as culture medium components and culture conditions, to ensure optimal expression and stability of the cells. We provide detailed training guides to help clients overcome potential issues.
A: Yes, our GFP Stable Cell Line-SK-OV-3 has undergone rigorous contaminant testing, including testing for common contaminants such as mycoplasma and bacteria. We use methods such as PCR and cell culture testing to ensure the purity of our cell lines. Our strict quality control procedures ensure that each batch of cell lines meets high standards of purity.
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During use, I found that the growth status of these cells was very stable, they always seemed to maintain a consistent growth rate, and their gene expression was also very stable. This is very important for my experiments as I need reliable cell models to conduct my research. The SK-OV-3 cell line not only provides stable cell growth, but also stable gene expression, which makes my experimental results more credible.
I used this SK-OV-3 GFP stable cell line to conduct a series of experiments and I was very satisfied with the results. The cells showed excellent stability in culture, with very consistent growth and stable gene expression. I observed that these cells exhibited bright green fluorescence under a fluorescence microscope, further demonstrating their stability and reliability.
I did some experiments with this SK-OV-3 GFP stable cell line and the results were very satisfactory. These cells showed very good stability during the culture process, their growth status has been very healthy and stable, and their gene expression has also been maintained at a stable level. I observed that these cells showed bright green fluorescence under a fluorescence microscope, which indicated that the GFP gene was well expressed in these cells.
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