GFP/Luciferase Reporter Stable Cell Line - SK-OV-3

Name: GFP/Luciferase Reporter Stable Cell Line - SK-OV-3
SKU: CSC-RR0060
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0060

Host Cell : SKOV-3 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RR0060
Introduction	The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. One famous example is the firefly luciferase (EC 1.13.12.7) from the firefly Photinus pyralis. Firefly luciferase as a laboratory reagent usually refers to P. pyralis luciferase although recombinant luciferases from several other species of fireflies are also commercially available.
Target Gene	GFP/Luciferase
Abbr	SKOV-3-GFP/Luciferase
Host Cell	SKOV-3
Host Cell Species	Homo sapiens (Human)
Reporter Type	Fluorescent protein
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Applications

Q & A

Customer Reviews

The GFP (Green Fluorescent Protein) gene, originally derived from the jellyfish Aequorea victoria, is widely used as a marker for protein localization and cell viability. Luciferase, an enzyme that produces light, is commonly used as a reporter gene to measure gene expression and cellular activity. The combination of GFP and luciferase in a single reporter system allows for the simultaneous monitoring of cell viability and gene expression.

The SKOV-3 cell line is a human ovarian cancer cell line that is often used in cancer research. The GFP/Luciferase reporter stable cell line in SKOV-3 provides a dual-reporter system for studying various biological processes, including gene expression, cell viability, and the effects of drugs or other treatments on these processes. This cell line is particularly useful for high-throughput screening and for studying the effects of cancer-related genes and their potential therapeutic targets.

The GFP/Luciferase Reporter Stable Cell Line - SKOV-3 is a dual-reporter cell line that expresses both green fluorescent protein (GFP) and luciferase. This system is useful for monitoring gene expression and protein activity in real-time.

(1)Gene Expression Studies: The GFP/Luciferase system in SKOV-3 cells can be used to study the regulation of gene expression under various experimental conditions, such as exposure to drugs, genetic mutations, or environmental stressors.

(2)Bioluminescence Imaging: The luciferase reporter allows for the use of bioluminescence imaging to track cell behavior in vivo, making this cell line ideal for preclinical studies of cancer metastasis, drug delivery, and therapeutic efficacy.

(3)Transcription Factor Activity: Researchers can use the GFP/Luciferase reporters to assess the activity of transcription factors in response to different stimuli, providing insights into the mechanisms of transcriptional regulation.

Customer Q&As

How does the GFP/Luciferase Reporter Stable Cell Line-SKOV-3 facilitate real-time monitoring of gene expression in ovarian cancer research?

A: This cell line allows for the non-invasive real-time monitoring of gene expression by detecting the bioluminescent signal from luciferase and the fluorescent signal from GFP. These signals can be quantified using a luminometer and a fluorescence microscope or plate reader, respectively, to monitor gene expression dynamically in ovarian cancer studies.

What are the advantages of having dual reporters (GFP and luciferase) in the GFP/Luciferase Reporter Stable Cell Line-SKOV-3 for drug screening assays?

A: The dual-reporter system enables concurrent monitoring of cell viability and reporter gene expression. GFP fluorescence can be used as a marker for cell health or transfection efficiency, while luciferase activity can indicate the activity of specific promoters or pathways targeted by drugs.

Can the GFP/Luciferase Reporter Stable Cell Line-SKOV-3 be utilized to assess the temporal effects of anticancer drugs on promoter activity specific to ovarian cancer?

A: Yes, by treating these cells with anticancer drugs and measuring luciferase activity at various time points, researchers can determine the timing and duration of promoter activation or repression in response to treatment.

How might the GFP/Luciferase Reporter Stable Cell Line-SKOV-3 be used to investigate the impact of microenvironmental factors on gene expression in ovarian cancer cells?

A: The cell line can be cultured under different microenvironmental conditions, such as hypoxia or in the presence of stromal cells. The effect of these conditions on gene expression can then be assessed by measuring GFP and luciferase signals, providing insights into how the tumor microenvironment influences ovarian cancer gene expression.

What experimental setup would be recommended for quantifying the effect of gene silencing on promoter-driven GFP and luciferase expression in the GFP/Luciferase Reporter Stable Cell Line-SKOV-3?

A: To quantify the effect of gene silencing, cells can be transfected with siRNAs targeting the gene of interest, followed by measurement of GFP and luciferase signals. A decrease in fluorescence and luminescence would indicate successful silencing and its consequent effect on promoter activity.

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Customer Reviews

Dual Reporter Genes

The GFP/Luciferase Reporter Stable Cell Line-SKOV-3 utilizes both GFP (Green Fluorescent Protein) and luciferase as reporters, enabling both qualitative and quantitative analysis. This dual functionality allows us to visually track cell behavior with GFP while quantitatively measuring cellular responses via luciferase activity.

United States

2023-08-25

High Expression Level

This cell line is engineered for high expression levels of both GFP and luciferase, ensuring that the fluorescent and luminescent signals are robust and easily detectable. High expression levels enhance the reliability and sensitivity of experimental assays involving these reporters.

United Kingdom

2022-07-26

Real-Time Monitoring

GFP allows for real-time, non-destructive observation of cellular processes without the need for cell lysis. This is particularly valuable for dynamic studies where continuous monitoring is essential, as in the GFP/Luciferase Reporter Stable Cell Line-SKOV-3.

Germany

2021-06-11

Stable Performance Over Time

The stability of GFP and luciferase expression in the GFP/Luciferase Reporter Stable Cell Line-SKOV-3 ensures consistent performance over the course of long-term experiments. This stability is crucial for obtaining reliable, reproducible results in prolonged studies.

United States

2022-01-26

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GFP/Luciferase Reporter Stable Cell Line - SK-OV-3

Cell Line Information

Cell Culture Information

Safety and Packaging

Background

Applications

Q & A

Customer Reviews

How does the GFP/Luciferase Reporter Stable Cell Line-SKOV-3 facilitate real-time monitoring of gene expression in ovarian cancer research?

What are the advantages of having dual reporters (GFP and luciferase) in the GFP/Luciferase Reporter Stable Cell Line-SKOV-3 for drug screening assays?

Can the GFP/Luciferase Reporter Stable Cell Line-SKOV-3 be utilized to assess the temporal effects of anticancer drugs on promoter activity specific to ovarian cancer?

How might the GFP/Luciferase Reporter Stable Cell Line-SKOV-3 be used to investigate the impact of microenvironmental factors on gene expression in ovarian cancer cells?

What experimental setup would be recommended for quantifying the effect of gene silencing on promoter-driven GFP and luciferase expression in the GFP/Luciferase Reporter Stable Cell Line-SKOV-3?

Dual Reporter Genes

High Expression Level

Real-Time Monitoring

Stable Performance Over Time

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