GFP/Luc Reporter Cell Line - RAW264.7

Name: GFP/Luc Reporter Cell Line - RAW264.7
SKU: CSC-RR00872
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR00872

Host Cell : RAW264.7 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RR00872
Description	This cell line is engineered to stably express GFP and luciferase reporter genes in RAW264.7 cells. It is an ideal cell line model in both fluorescent and bioluminescent tracking of RAW264.7 cells.
Product Type	Stable cell line constitutively co-expressing GFP and luciferase reporter genes
Target Gene	GFP/Luc
Host Cell	RAW264.7
Host Cell Species	Mus musculus (Mouse)
Applications	1) in vitro cell tracking by fluorescent and bioluminescent signal 2) monitor in vivo tumor growth using the fluorescence and bioluminescence signal 3) anticancer drug development
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	1) cell line imaging under fluorescent microscopy 2) in vitro cell-based luciferase assay 2) mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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