RFP Reporter Cell Line - RAW264.7

Name: RFP Reporter Cell Line - RAW264.7
SKU: CSC-RR00823
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR00823

Host Cell : RAW264.7 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RR00823
Description	This cell line is engineered to stably express RFP reporter gene in RAW264.7 cells. It is an ideal cell line model in fluorescent tracking of RAW264.7 cells.
Product Type	Stable cell line constitutively expressing RFP reporter gene
Target Gene	RFP
Host Cell	RAW264.7
Host Cell Species	Mus musculus (Mouse)
Applications	1) in vitro cell tracking by fluorescent signal 2) monitor in vivo tumor growth using the fluorescence signal 3) anticancer drug development
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	1) cell line imaging under fluorescent microscopy 2) mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

RFP

Background

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008]

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