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Cat.No. : CSC-RO0909 Host Cell: MIA PaCa-2
| Cat. No. | CSC-RO0909 |
| Description | This cell line is engineered to stably overexpress human CD274 (also named as PDL1). |
| Gene | CD274 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | MIA PaCa-2 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: This cell line was obtained by transfecting the human CD274 (PD-L1) gene into MiaPaCa2 pancreatic cancer cells and achieving stable gene expression.
A: It is primarily used for studying the role of PD-L1 in tumor immune evasion and anti-tumor drug development.
A: Attention should be paid to the culture environment, temperature, CO2 concentration, and choice of culture medium to maintain cell stability and viability.
A: It can be used for screening and evaluating antibody drugs targeting PD-L1, particularly in cancer treatment.
A: Due to the importance of PD-L1 in tumor immunity, this cell line is particularly significant for studying the regulatory mechanisms of the PD-1/PD-L1 signaling pathway.
A: It can be used to simulate and study changes in PD-L1 expression in the tumor microenvironment and its impact on tumor cell immune evasion.
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