Human CD274 Stable Cell Line - MIA PaCa-2

Name: Human CD274 Stable Cell Line - MIA PaCa-2
SKU: CSC-RO0909
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0909

Host Cell : MIA PaCa-2 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0909
Description	This cell line is engineered to stably overexpress human CD274 (also named as PDL1).
Target Gene	CD274
Gene Species	Homo sapiens (Human)
Host Cell	MIA PaCa-2
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD274 CD274 molecule [ Homo sapiens ]
Gene Symbol	CD274
Synonyms	CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296;
Gene ID	29126
Uni Prot ID	Q9NZQ7
m RNA Refseq	BC113734
Chromosome Location	9p24.1
Function	protein binding; protein tyrosine phosphatase activity; receptor activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; PD-1 signaling, organism-specific biosystem;
MIM	605402

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Q & A

Customer Reviews

Customer Q&As

What is the origin of the Human CD274 Stable Cell Line - MiaPaCa2?

A: This cell line was obtained by transfecting the human CD274 (PD-L1) gene into MiaPaCa2 pancreatic cancer cells and achieving stable gene expression.

What are the main research applications of the Human CD274 Stable Cell Line - MiaPaCa2?

A: It is primarily used for studying the role of PD-L1 in tumor immune evasion and anti-tumor drug development.

What considerations should be made when culturing the Human CD274 Stable Cell Line - MiaPaCa2?

A: Attention should be paid to the culture environment, temperature, CO2 concentration, and choice of culture medium to maintain cell stability and viability.

What is the role of the Human CD274 Stable Cell Line - MiaPaCa2 in drug screening?

A: It can be used for screening and evaluating antibody drugs targeting PD-L1, particularly in cancer treatment.

What is the significance of the Human CD274 Stable Cell Line - MiaPaCa2 in cell signaling research?

A: Due to the importance of PD-L1 in tumor immunity, this cell line is particularly significant for studying the regulatory mechanisms of the PD-1/PD-L1 signaling pathway.

How is the Human CD274 Stable Cell Line - MiaPaCa2 applied in simulating the tumor microenvironment?

A: It can be used to simulate and study changes in PD-L1 expression in the tumor microenvironment and its impact on tumor cell immune evasion.

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