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Cat.No. : CSC-RR0322 Host Cell: MIA PaCa-2
| Cat. No. | CSC-RR0322 |
| Description | MIA PaCa2-Luciferase cell line stably overexpresses luciferase as a reporter of MIA PaCa2 cells. It is a good ready-to-use cell model for tracing MIA PaCa2 cells. |
| Gene | Luciferase |
| Host Cell | MIA PaCa-2 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Source | MIA PaCa-2 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Mostly because of resistance to chemotherapy, especially gemcitabine, pancreatic cancer (PC) is notoriously difficult to treat. Emerging as a key gene linked with this resistance is the ribonucleotide reductase subunit M2 (RRM2). Targeting RRM2, researchers sought to find microRNAs that would boost gemcitabine sensitivity. Through MTT assays and Western blot analyses, they confirmed that prolonged gemcitabine treatment increased RRM2 expression in PC cells, leading to drug resistance. Among the potential microRNAs, miR-20a-5p was identified as a significant regulator, effectively targeting RRM2's 3′UTR and reversing resistance in PC cells. Retrospective clinical studies indicated a strong correlation between miR-20a-5p levels and patient responses to gemcitabine therapy, suggesting its potential as a predictive biomarker for treatment efficacy.
Figure 1. The researchers evaluated gemcitabine resistance in MIA-PaCa2 and MIA-PaCa2-GEM cells using MTT assays, FACS analysis, and qRT-PCR for RRM2 expression. (Lu H, et al., 2019)
The MIA PaCa2 cell line, used in these studies, is valuable for exploring the mechanisms of drug resistance and testing novel therapeutic strategies. Creative Biogene's Luciferase Reporter Cell Line-MIA PaCa2 is designed to facilitate similar research directions. This cell line enables researchers to monitor RRM2 expression and validate the impact of miR-20a-5p or other regulatory factors on gemcitabine sensitivity. By employing the luciferase reporter system, scientists can gain insights into gene regulation and enhance the understanding of pancreatic cancer treatment approaches.
A: The MIA PaCa2 cell line engineered to stably express a luciferase reporter gene provides a unique opportunity to study the molecular mechanisms of pancreatic cancer in a way that allows for real-time, non-invasive monitoring of tumor growth and response to therapeutic interventions. This cell line maintains the aggressive and metastatic properties of the parental MIA PaCa2 cells, which is crucial for modeling the disease accurately. The luciferase gene integration enables researchers to track cellular processes through bioluminescent imaging, facilitating the study of gene expression, tumor progression, and the evaluation of novel therapeutic strategies.
A: To optimize the use of the MIA PaCa2 Luciferase Reporter Cell Line in xenograft models, researchers should first establish the appropriate cell passage and engraftment conditions. Once the xenografts are established, they can administer the test compounds and monitor tumor growth and response over time using bioluminescent imaging. The key is to perform these assays under controlled conditions, ensuring consistent and reliable data collection. Additionally, researchers should consider using multiple imaging time points and correlating the bioluminescent signal with histological and molecular analyses to comprehensively assess the efficacy of the anti-cancer agents.
A: When designing experiments to investigate gene function in the context of pancreatic cancer using the MIA PaCa2 Luciferase Reporter Cell Line, it is essential to select appropriate genetic manipulation techniques, such as CRISPR/Cas9 or lentiviral vectors for gene overexpression or knockdown. Researchers must also consider the timing and duration of these genetic interventions, as well as the appropriate controls to ensure that any observed changes in luciferase activity are directly attributable to the manipulation of the gene of interest. Moreover, it is crucial to validate the efficiency of gene editing or overexpression and to perform replicate experiments to ensure the reliability of the results.
A: The MIA PaCa2 Luciferase Reporter Cell Line enables researchers to track the behavior of pancreatic cancer cells in vitro and in vivo, providing insights into the molecular mechanisms that drive tumor progression and metastasis. By monitoring changes in luciferase activity, researchers can assess the effects of genetic alterations, signaling pathways, and environmental factors on cancer cell behavior. This information is invaluable for identifying potential therapeutic targets and for developing strategies to prevent or treat the spread of pancreatic cancer.
A: The MIA PaCa2 Luciferase Reporter Cell Line can be used to generate patient-derived xenograft (PDX) models, which closely resemble the genetic and phenotypic characteristics of the original tumor. These models can then be utilized to test the efficacy of personalized treatment regimens, allowing researchers to evaluate the response of individual tumors to various therapeutic agents. By using this cell line in PDX models, researchers can contribute to the advancement of personalized medicine in pancreatic cancer treatment, potentially leading to more effective and tailored therapies for patients.
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The cell line's high-sensitivity luciferase allows for the detection of minimal promoter activity changes, critical for understanding gene regulation in the Luciferase Reporter Cell Line - MIA PaCa2.
Germany
10/11/2023
This cell line enables quantitative analysis of bioluminescent signals, offering precise measurements for gene expression studies in the Luciferase Reporter Cell Line - MIA PaCa2.
French
05/13/2020
Luciferase assays in the Luciferase Reporter Cell Line - MIA PaCa2 are non-destructive, allowing for serial measurements from the same cells over time, enhancing longitudinal study designs.
Germany
03/26/2023
With the luciferase reporter, the Luciferase Reporter Cell Line - MIA PaCa2 provides rapid assay results, accelerating the research cycle from experimental setup to data collection.
United Kingdom
05/25/2020
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